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Literature summary for 1.1.98.2 extracted from

  • Oyugi, M.A.; Bashiri, G.; Baker, E.N.; Johnson-Winters, K.
    Investigating the reaction mechanism of F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium tuberculosis: Kinetic analysis of the wild-type and mutant enzymes (2016), Biochemistry, 55, 5566-5577.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
E109Q the mutant has 60fold decreased catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
H40A the mutant has 1000fold decreased catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
W44A the mutant has 2000fold decreased catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
W44F the mutant has 2fold increased catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis
W44Y the mutant has 34fold decreased catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
oxidized coenzyme F420 mutant enzyme W44A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.0026
-
oxidized coenzyme F420 wild type enzyme, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.0042
-
oxidized coenzyme F420 mutant enzyme W44Y, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.0054
-
oxidized coenzyme F420 mutant enzyme W44F, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.0056
-
oxidized coenzyme F420 mutant enzyme E109Q, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.008
-
oxidized coenzyme F420 mutant enzyme H40A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.022
-
D-glucose 6-phosphate wild type enzyme, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.027
-
D-glucose 6-phosphate mutant enzyme H40A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.041
-
D-glucose 6-phosphate mutant enzyme E109Q, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.17
-
D-glucose 6-phosphate mutant enzyme W44Y, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.28
-
D-glucose 6-phosphate mutant enzyme W44A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.34
-
D-glucose 6-phosphate mutant enzyme W44F, at pH 7.0 and 22°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + oxidized coenzyme F420 Mycobacterium tuberculosis
-
6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis Q0RVH7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + oxidized coenzyme F420
-
Mycobacterium tuberculosis 6-phospho-D-glucono-1,5-lactone + reduced coenzyme F420
-
?

Synonyms

Synonyms Comment Organism
F420-dependent glucose-6-phosphate dehydrogenase
-
Mycobacterium tuberculosis
FGD
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
oxidized coenzyme F420 mutant enzyme W44A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.0012
-
D-glucose 6-phosphate mutant enzyme W44A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.0016
-
D-glucose 6-phosphate mutant enzyme H40A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.002
-
oxidized coenzyme F420 mutant enzyme H40A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.02
-
oxidized coenzyme F420 mutant enzyme E109Q, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.023
-
D-glucose 6-phosphate mutant enzyme E109Q, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.041
-
D-glucose 6-phosphate mutant enzyme W44Y, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.06
-
oxidized coenzyme F420 mutant enzyme W44Y, at pH 7.0 and 22°C Mycobacterium tuberculosis
1.4
-
D-glucose 6-phosphate wild type enzyme, at pH 7.0 and 22°C Mycobacterium tuberculosis
1.4
-
oxidized coenzyme F420 wild type enzyme, at pH 7.0 and 22°C Mycobacterium tuberculosis
3.2
-
oxidized coenzyme F420 mutant enzyme W44F, at pH 7.0 and 22°C Mycobacterium tuberculosis
3.5
-
D-glucose 6-phosphate mutant enzyme W44F, at pH 7.0 and 22°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
coenzyme F420
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.004
-
D-glucose 6-phosphate mutant enzyme W44A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.06
-
D-glucose 6-phosphate mutant enzyme H40A, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.1
-
D-glucose 6-phosphate mutant enzyme E109Q, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.2
-
D-glucose 6-phosphate mutant enzyme W44Y, at pH 7.0 and 22°C Mycobacterium tuberculosis
0.4
-
oxidized coenzyme F420 mutant enzyme H40A, at pH 7.0 and 22°C Mycobacterium tuberculosis
1
-
oxidized coenzyme F420 mutant enzyme W44A, at pH 7.0 and 22°C Mycobacterium tuberculosis
4
-
oxidized coenzyme F420 mutant enzyme E109Q, at pH 7.0 and 22°C Mycobacterium tuberculosis
10
-
D-glucose 6-phosphate mutant enzyme W44F, at pH 7.0 and 22°C Mycobacterium tuberculosis
20
-
oxidized coenzyme F420 mutant enzyme W44Y, at pH 7.0 and 22°C Mycobacterium tuberculosis
60
-
D-glucose 6-phosphate wild type enzyme, at pH 7.0 and 22°C Mycobacterium tuberculosis
600
-
oxidized coenzyme F420 wild type enzyme, at pH 7.0 and 22°C Mycobacterium tuberculosis
600
-
oxidized coenzyme F420 mutant enzyme W44F, at pH 7.0 and 22°C Mycobacterium tuberculosis