Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
oxidized coenzyme F420 | pH 7.0, 40°C | Mycolicibacterium smegmatis | |
1.6 | - |
D-glucose 6-phosphate | pH 7, 40°C | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
78000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + oxidized coenzyme F420 | Mycolicibacterium smegmatis | there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49) | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, phenyl-Sepharose column chromatography, F420ether-linked aminohexylSepharose 4B affinity chromatography, and quaternary aminoethyl-Sephadex column chromatography | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50.9 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
4°C, 16 days, highly purified enzyme, retention of 60% and 20% of the original activity with and without glycerol, respectively | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose-6-phosphate + oxidized coenzyme F420 | 9% of the activity with 2 mM D-glucose 6-phosphate | Mycolicibacterium smegmatis | ? | - |
? | |
D-glucose 6-phosphate + oxidized coenzyme F420 | there are to separate glucose-6-phosphate dehydrogenase activities in Mycobacterium smegmatis: coenzyme F420-dependent glucose-6-phosphate dehydrogenase and NADP+-dependent glucose-6-phosphate dehydrogenase (EC 1.1.1.49) | Mycolicibacterium smegmatis | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
D-glucose 6-phosphate + oxidized coenzyme F420 | the purified enzyme used F420 as its obligate electron acceptor when D-glucose 6-phosphate is the substrate. NAD, NADP, flavin adenine dinucleotide, and flavin mononucleotide show no reactivity. The enzyme is very specific for D-glucose 6-phosphate. At pH 7.0 and 22°C, 10 mM D-mannose-6-phosphate and D-fructose-6-phosphate show 5% and 9%, respectively, of the activity with 2 mM D-glucose 6-phosphate. No activity with: D-fructose-1-phosphate, D-galactose-1-phosphate, D-galactose-6-phosphate, L-fucose-1-phosphate, D-glucose-1-phosphate, D-ribose-5-phosphate, D-ribose-1-phosphate, D-arabinose-5-phosphate, glyceraldehyde-3-phosphate, and glycerol-3-phosphate | Mycolicibacterium smegmatis | D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420 | - |
? | |
D-mannose-6-phosphate + oxidized coenzyme F420 | 5% of the activity with 2 mM D-glucose 6-phosphate | Mycolicibacterium smegmatis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
coenzyme F420-dependent glucose-6-phosphate dehydrogenase | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mycolicibacterium smegmatis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
denaturation above | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
maxima at pH 5.5 and pH 8.0 | Mycolicibacterium smegmatis |
8 | - |
maxima at pH 5.5 and pH 8.0 | Mycolicibacterium smegmatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5: about 60% of maximal activity, pH 5.5: maximum, pH 7: about 50% of maximal activity, pH 8.0: maximal activity, pH 8.5: about 60% of maximal activity | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | - |
Mycolicibacterium smegmatis |