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Literature summary for 1.1.9.1 extracted from

  • Toyama, H.; Fujii, A.; Matsushita, K.; Shinagawa, E.; Ameyama, M.; Adachi, O.
    Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols (1995), J. Bacteriol., 177, 2442-2450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.9.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.105
-
 1-butanol isoform ADH IIB, pH 8.0 Pseudomonas putida
0.15
-
 1-butanol isoform ADH IIG, pH 8.0 Pseudomonas putida
0.226
-
 1,2-propanediol isoform ADH IIG, pH 8.0 Pseudomonas putida
2.4
-
 glycerol isoform ADH IIG, pH 8.0 Pseudomonas putida
3 3.2 ethanol isoform ADH IIG, pH 8.0 Pseudomonas putida
5.61
-
 ethanol isoform ADH IIB, pH 8.0 Pseudomonas putida
10.2
-
 1,2-propanediol isoform ADH IIB, pH 8.0 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE Pseudomonas putida
72000
-
 ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14
-
 isoform ADH IIG, pH 9.0 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-butanediol + ferricyanide ADH IIB, 84% of the activity with 1-butanol. ADH IIG, 86% of the activity with 1,2-propanediol Pseudomonas putida 2-hydroxybutanal + ferrocyanide
-
 ?
1,2-ethanediol + ferricyanide ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol Pseudomonas putida hydroxyacetaldehyde + ferrocyanide
-
 ?
1,2-propanediol + ferricyanide ADH IIB, 43% of the activity with 1-butanol. ADH IIG, maximum activity Pseudomonas putida 2-hydroxypropanal + ferrocyanide
-
 ?
1,3-butanediol + ferricyanide ADH IIB, 105% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxybutanal + ferrocyanide
-
 ?
1,3-propandione + ferricyanide ADH IIB, 61% of the activity with 1-butanol. ADH IIG, 42% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxypropanal + ferrocyanide
-
 ?
1,4-butanediol + ferricyanide ADH IIB, 110% of the activity with 1-butanol. ADH IIG, 75% of the activity with 1,2-propanediol Pseudomonas putida 4-hydroxybutanal + ferrocyanide
-
 ?
1-butanol + ferricyanide ADH IIG, 42% of the activity with 1,2-propanediol Pseudomonas putida butanal + ferrocyanide
-
 ?
1-heptanol + ferricyanide ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 39% of the activity with 1,2-propanediol Pseudomonas putida heptanal + ferrocyanide
-
 ?
1-hexanol + ferricyanide ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol Pseudomonas putida hexanal + ferrocyanide
-
 ?
1-octanol + ferricyanide ADH IIB, 44% of the activity with 1-butanol. ADH IIG, 24% of the activity with 1,2-propanediol Pseudomonas putida octanal + ferrocyanide
-
 ?
1-pentanol + ferricyanide ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 40% of the activity with 1,2-propanediol Pseudomonas putida pentanal + ferrocyanide
-
 ?
1-propanol + ferricyanide ADH IIB, 70% of the activity with 1-butanol. ADH IIG, 41% of the activity with 1,2-propanediol Pseudomonas putida propanal + ferrocyanide
-
 ?
2,3-butanediol + ferricyanide ADH IIB, 12% of the activity with 1-butanol. ADH IIG, 71% of the activity with 1,2-propanediol Pseudomonas putida 3-hydroxybutan-2-one + ferrocyanide
-
 ?
2,4-pentanediol + ferricyanide ADH IIB, 45% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol Pseudomonas putida 4-hydroxypentan-2-one + ferrocyanide
-
 ?
2-butanol + ferricyanide ADH IIB, 90% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol Pseudomonas putida butanone + ferrocyanide
-
 ?
2-hexanol + ferricyanide ADH IIB, 99% of the activity with 1-butanol. ADH IIG, 33% of the activity with 1,2-propanediol Pseudomonas putida hexan-2-one + ferrocyanide
-
 ?
2-pentanol + ferricyanide ADH IIB, 120% of the activity with 1-butanol. ADH IIG, 19% of the activity with 1,2-propanediol Pseudomonas putida pentan-2-one + ferrocyanide
-
 ?
2-propanol + ferricyanide ADH IIB, 52% of the activity with 1-butanol. ADH IIG, 0% of the activity with 1,2-propanediol Pseudomonas putida acetone + ferrocyanide
-
 ?
3-pentanol + ferricyanide ADH IIB, 43% of the activity with 1-butanol. ADH IIG, 15% of the activity with 1,2-propanediol Pseudomonas putida pentan-3-one + ferrocyanide
-
 ?
allyl alcohol + ferricyanide ADH IIB, 160% of the activity with 1-butanol. ADH IIG, 46% of the activity with 1,2-propanediol Pseudomonas putida prop-2-enal + ferrocyanide
-
 ?
butanal + ferrocyanide ADH IIB, 32% of the activity with 1-butanol. ADH IIG, 13% of the activity with 1,2-propanediol Pseudomonas putida 1-butanol + ferricyanide
-
 ?
ethanal + ferrocyanide ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 16% of the activity with 1,2-propanediol Pseudomonas putida ethanol + ferricyanide
-
 ?
ethanol + ferricyanide ADH IIB, 78% of the activity with 1-butanol. ADH IIG, 22% of the activity with 1,2-propanediol Pseudomonas putida ethanal + ferrocyanide
-
 ?
glycerol + ferrocyanide ADH IIB, 0% of the activity with 1-butanol. ADH IIG, 63% of the activity with 1,2-propanediol Pseudomonas putida 2,3-dihydroxypropanal + ferricyanide
-
 ?
iso-amyl alcohol + ferricyanide ADH IIB, 100% of the activity with 1-butanol. ADH IIG, 93% of the activity with 1,2-propanediol Pseudomonas putida 3-methylbutanol + ferrocyanide
-
 ?
propanal + ferrocyanide ADH IIB, 125% of the activity with 1-butanol. ADH IIG, 58% of the activity with 1,2-propanediol Pseudomonas putida 1-propanol + ferricyanide
-
 ?

Subunits

Subunits Comment Organism
monomer ADH IIB, 1 * 69000, SDS-PAGE. ADH IIG, 1 * 72000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
ADH IIB
-
 Pseudomonas putida
ADH IIG
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
heme c both isoforms ADH IIB and ADH IIG Pseudomonas putida
pyrroloquinoline quinone both isoforms ADH IIB and ADH IIG Pseudomonas putida

General Information

General Information Comment Organism
metabolism ADH IIB is formed in the cells grown on alcohols of medium chain length and also on 1,3-butanediol. Induction of ADH IIG is restricted to 1,2-propanediol or glycerol, of which the former alcohol is more effective Pseudomonas putida