Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | myo-inositol induces the enzyme maximally at 33 mM, but is slightly suppressing at higher concentrations, allo-inositol, muco-inositol, and D-sorbitol also induces the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview | Gluconobacter oxydans |
Cloned (Comment) | Organism |
---|---|
gene GOX1857, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli | Gluconobacter oxydans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a GOX1857 disruption mutant strain DW1, which is inactive with myo-inositol and unable to grow with myo-inositol as the sole energy source | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | D-glucose suppresses the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Gluconobacter oxydans | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
strain ATCC 621H, DSM 2343, gene GOX1857 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
D-sorbitol induced wild-type strain, crude extract | Gluconobacter oxydans |
0.6 | - |
membrane fraction of induced wild-type cells | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allo-inositol + 2,6-dichlorophenol indophenol | i.e. 1,2,3,4/5,6-cyclohexanehexol | Gluconobacter oxydans | ? | - |
? | |
D-sorbitol + 2,6-dichlorophenol indophenol | - |
Gluconobacter oxydans | ? | - |
? | |
additional information | substrate specificity, overview | Gluconobacter oxydans | ? | - |
? | |
muco-inositol + 2,6-dichlorophenol indophenol | i.e. 1,2,4,5/3,6-cyclohexanehexol | Gluconobacter oxydans | ? | - |
? | |
myo-inositol + 2,6-dichlorophenol indophenol | - |
Gluconobacter oxydans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
membrane-bound quinoprotein inositol dehydrogenase | - |
Gluconobacter oxydans |
QIDH | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | 8.8 | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | dependent on | Gluconobacter oxydans |