BRENDA - Enzyme Database
show all sequences of 1.1.5.B1

Identification of membrane-bound quinoprotein inositol dehydrogenase in Gluconobacter oxydans ATCC 621H

Hoelscher, T.; Weinert-Sepalage, D.; Goerisch, H.; Microbiology 153, 499-506 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
myo-inositol induces the enzyme maximally at 33 mM, but is slightly suppressing at higher concentrations, allo-inositol, muco-inositol, and D-sorbitol also induces the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview
Gluconobacter oxydans
Cloned(Commentary)
Cloned (Commentary)
Organism
gene GOX1857, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli
Gluconobacter oxydans
Engineering
Protein Variants
Commentary
Organism
additional information
construction of a GOX1857 disruption mutant strain DW1, which is inactive with myo-inositol and unable to grow with myo-inositol as the sole energy source
Gluconobacter oxydans
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
D-glucose suppresses the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Gluconobacter oxydans
16020
-
Organism
Organism
UniProt
Commentary
Textmining
Gluconobacter oxydans
-
strain ATCC 621H, DSM 2343, gene GOX1857
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.09
-
D-sorbitol induced wild-type strain, crude extract
Gluconobacter oxydans
0.6
-
membrane fraction of induced wild-type cells
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
allo-inositol + 2,6-dichlorophenol indophenol
i.e. 1,2,3,4/5,6-cyclohexanehexol
688870
Gluconobacter oxydans
?
-
-
-
?
D-sorbitol + 2,6-dichlorophenol indophenol
-
688870
Gluconobacter oxydans
?
-
-
-
?
additional information
substrate specificity, overview
688870
Gluconobacter oxydans
?
-
-
-
?
muco-inositol + 2,6-dichlorophenol indophenol
i.e. 1,2,4,5/3,6-cyclohexanehexol
688870
Gluconobacter oxydans
?
-
-
-
?
myo-inositol + 2,6-dichlorophenol indophenol
-
688870
Gluconobacter oxydans
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
membrane-bound quinoprotein inositol dehydrogenase
-
Gluconobacter oxydans
QIDH
-
Gluconobacter oxydans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
8.8
-
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
dependent on
Gluconobacter oxydans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
myo-inositol induces the enzyme maximally at 33 mM, but is slightly suppressing at higher concentrations, allo-inositol, muco-inositol, and D-sorbitol also induces the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview
Gluconobacter oxydans
Cloned(Commentary) (protein specific)
Commentary
Organism
gene GOX1857, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyrroloquinoline quinone
dependent on
Gluconobacter oxydans
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of a GOX1857 disruption mutant strain DW1, which is inactive with myo-inositol and unable to grow with myo-inositol as the sole energy source
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
D-glucose suppresses the enzyme, D-mannitol suppresses enzyme induction by myo-inositol, overview
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
bound
Gluconobacter oxydans
16020
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.09
-
D-sorbitol induced wild-type strain, crude extract
Gluconobacter oxydans
0.6
-
membrane fraction of induced wild-type cells
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
allo-inositol + 2,6-dichlorophenol indophenol
i.e. 1,2,3,4/5,6-cyclohexanehexol
688870
Gluconobacter oxydans
?
-
-
-
?
D-sorbitol + 2,6-dichlorophenol indophenol
-
688870
Gluconobacter oxydans
?
-
-
-
?
additional information
substrate specificity, overview
688870
Gluconobacter oxydans
?
-
-
-
?
muco-inositol + 2,6-dichlorophenol indophenol
i.e. 1,2,4,5/3,6-cyclohexanehexol
688870
Gluconobacter oxydans
?
-
-
-
?
myo-inositol + 2,6-dichlorophenol indophenol
-
688870
Gluconobacter oxydans
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.7
8.8
-
Gluconobacter oxydans
Other publictions for EC 1.1.5.B1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
688870
Hoelscher
Identification of membrane-bou ...
Gluconobacter oxydans
Microbiology
153
499-506
2007
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