Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Burkholderia cepacia |
Protein Variants | Comment | Organism |
---|---|---|
S326C | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S326E | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S326G | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S326H | the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme | Burkholderia cepacia |
S326K | the mutant shows increased activity toward D-glucose compared to the wild type enzyme | Burkholderia cepacia |
S326Q | the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme | Burkholderia cepacia |
S326Q/S365Y | the mutant is virtually non-reactive to maltose while retaining high D-glucose dehydrogenase activity | Burkholderia cepacia |
S326R | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S326T | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S326V | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S326Y | the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme | Burkholderia cepacia |
S365A | the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme | Burkholderia cepacia |
S365C | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365D | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365E | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365F | the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme | Burkholderia cepacia |
S365G | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365H | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365I | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365K | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365L | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365M | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365N | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365P | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365Q | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365R | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365T | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365V | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365W | the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365Y | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y | the mutant shows strongly reduced activity toward D-glucose and maltose compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326E | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326G | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326H | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326K | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326Q | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326R | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326T | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
S365Y/S326V | the mutant shows reduced activity compared to the wild type enzyme | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-5PW column chromatography and Superdex-200 gel filtration | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD glucose dehydrogenase | - |
Burkholderia cepacia |
FADGDH | - |
Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Burkholderia cepacia |