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Literature summary for 1.1.5.9 extracted from

  • Yamashita, Y.; Ferri, S.; Huynh, M.L.; Shimizu, H.; Yamaoka, H.; Sode, K.
    Direct electron transfer type disposable sensor strip for glucose sensing employing an engineered FAD glucose dehydrogenase (2013), Enzyme Microb. Technol., 52, 123-128.
    View publication on PubMed
Search for more literature for 1.1.5.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Burkholderia cepacia

Protein Variants

Protein Variants Comment Organism
S326C the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S326E the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S326G the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S326H the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
S326K the mutant shows increased activity toward D-glucose compared to the wild type enzyme Burkholderia cepacia
S326Q the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
S326Q/S365Y the mutant is virtually non-reactive to maltose while retaining high D-glucose dehydrogenase activity Burkholderia cepacia
S326R the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S326T the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S326V the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S326Y the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
S365A the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
S365C the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365D the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365E the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365F the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
S365G the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365H the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365I the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365K the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365L the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365M the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365N the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365P the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365Q the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365R the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365T the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365V the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365W the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365Y the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y the mutant shows strongly reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
S365Y/S326E the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y/S326G the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y/S326H the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y/S326K the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y/S326Q the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y/S326R the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y/S326T the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
S365Y/S326V the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-5PW column chromatography and Superdex-200 gel filtration Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
-
 Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
maltose + 2,6-dichlorophenolindophenol
-
 Burkholderia cepacia maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

Synonyms Comment Organism
FAD glucose dehydrogenase
-
 Burkholderia cepacia
FADGDH
-
 Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Burkholderia cepacia