Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Origami2(DE3) | Aspergillus flavus |
Protein Variants | Comment | Organism |
---|---|---|
V149C/G190C | the mutant shows a 110 min half-life of thermal inactivation at 45°C, which is 13fold greater than that of the wild type enzyme | Aspergillus flavus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
D-glucose | mutant enzyme V149C7G190C, at pH 6.5 and 25°C | Aspergillus flavus | |
35 | - |
D-glucose | wild type enzyme, at pH 6.5 and 25°C | Aspergillus flavus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Aspergillus flavus |
61600 | - |
- |
Aspergillus flavus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | B8MX95 | - |
- |
Purification (Comment) | Organism |
---|---|
resource Q column chromatography | Aspergillus flavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | 100% activity | Aspergillus flavus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | 17% activity compared to D-glucose | Aspergillus flavus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | less than 1% activity with D-mannose, D-maltose, D-galactose, D-allose, D-lactose, D-fructose, sucrose, and cellobiose | Aspergillus flavus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Aspergillus flavus |
? | x * 61600, calculated from amino acid sequence | Aspergillus flavus |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent glucose dehydrogenase | - |
Aspergillus flavus |
FAD-GDH | - |
Aspergillus flavus |
FAD-glucose dehydrogenase | - |
Aspergillus flavus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the wild type enzyme is inactivated rapidly at 45°C, with less than 20% of the initial activity remaining after 25 min and a half-life of inactivation of less than 10 min | Aspergillus flavus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
760 | - |
D-glucose | mutant enzyme V149C7G190C, at pH 6.5 and 25°C | Aspergillus flavus | |
780 | - |
D-glucose | wild type enzyme, at pH 6.5 and 25°C | Aspergillus flavus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aspergillus flavus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
D-glucose | mutant enzyme V149C7G190C, at pH 6.5 and 25°C | Aspergillus flavus | |
22 | - |
D-glucose | wild type enzyme, at pH 6.5 and 25°C | Aspergillus flavus |