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Literature summary for 1.1.5.9 extracted from

  • Ozawa, K.; Iwasa, H.; Sasaki, N.; Kinoshita, N.; Hiratsuka, A.; Yokoyama, K.
    Identification and characterization of thermostable glucose dehydrogenases from thermophilic filamentous fungi (2017), Appl. Microbiol. Biotechnol., 101, 173-183.
    View publication on PubMed
Search for more literature for 1.1.5.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and Pichia pastoris Rasamsonia emersonii
expressed in Escherichia coli and Pichia pastoris Thermoascus crustaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Thermoascus crustaceus
60000
-
 x * 60000, SDS-PAGE Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii A0A1E1GL61
-
-
Rasamsonia emersonii NBRC 31232 A0A1E1GL61
-
-
Thermoascus crustaceus A0A1E1GL87
-
-
Thermoascus crustaceus NBRC 9129 A0A1E1GL87
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rasamsonia emersonii
glycoprotein
-
 Thermoascus crustaceus

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Rasamsonia emersonii
Ni-NTA agarose column chromatography Thermoascus crustaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 11.5% activity compared to D-glucose Rasamsonia emersonii 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 60.1% activity compared to D-glucose Thermoascus crustaceus 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 60.1% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
2-deoxy-D-glucose + 2,6-dichlorophenolindophenol 11.5% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 2-deoxy-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Rasamsonia emersonii D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Thermoascus crustaceus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Thermoascus crustaceus NBRC 9129 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-glucose + 2,6-dichlorophenolindophenol high substrate specificity for D-glucose Rasamsonia emersonii NBRC 31232 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-xylose + 2,6-dichloroindophenol 24.8% activity compared to D-glucose Rasamsonia emersonii D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol
-
 ?
D-xylose + 2,6-dichloroindophenol 24.8% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 D-xylono-1,5-lactone + 2,6-dichlorophenolindophenol
-
 ?
D-xylose + 2,6-dichlorophenolindophenol 27.6% activity compared to D-glucose Thermoascus crustaceus D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
D-xylose + 2,6-dichlorophenolindophenol 27.6% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
maltotriose + 2,6-dichlorophenolindophenol 3.4% activity compared to D-glucose Rasamsonia emersonii maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
maltotriose + 2,6-dichlorophenolindophenol 6.8% activity compared to D-glucose Thermoascus crustaceus maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
maltotriose + 2,6-dichlorophenolindophenol 6.8% activity compared to D-glucose Thermoascus crustaceus NBRC 9129 maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
maltotriose + 2,6-dichlorophenolindophenol 3.4% activity compared to D-glucose Rasamsonia emersonii NBRC 31232 maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Rasamsonia emersonii ?
-
 ?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Thermoascus crustaceus ?
-
 ?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Thermoascus crustaceus NBRC 9129 ?
-
 ?
additional information no activity in the presence of maltose, D-arabinose, D-fructose, D-galactose, maltotetraose, D-mannitol, D-mannose, D-raffinose, D-sorbitol, sucrose, and D-trehalose Rasamsonia emersonii NBRC 31232 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Rasamsonia emersonii
? x * 58000, SDS-PAGE Thermoascus crustaceus

Synonyms

Synonyms Comment Organism
FAD-dependent glucose dehydrogenase
-
 Rasamsonia emersonii
FAD-dependent glucose dehydrogenase
-
 Thermoascus crustaceus
FAD-GDH
-
 Rasamsonia emersonii
FAD-GDH
-
 Thermoascus crustaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
 Thermoascus crustaceus
65 70
-
 Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 after heat treatment at 40-70°C for 15 min, unglycosylated enzyme maintains nearly 100% activity at temperatures up to 45°C, but completely loses activity at 60°C, while glycosylated enzyme displays high thermostability, maintaining 89% activity at 60°C. The melting temperature of the glycosylated enzyme is 66.4°C Rasamsonia emersonii
40 62 unglycosylated enzym e is stable at temperatures up to 45°C , while glycosylatedenzyme maintains 75% activity at 60°C. The melting temperature of the glycosylated enzyme is 62.5°C Thermoascus crustaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rasamsonia emersonii
5
-
-
 Thermoascus crustaceus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 9 the enzyme remains stable between pH 2.0 and 9.0 Rasamsonia emersonii
2 9 the enzyme remains stable between pH 2.0 and 9.0 Thermoascus crustaceus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rasamsonia emersonii
FAD
-
 Thermoascus crustaceus