Cloned (Comment) | Organism |
---|---|
recombinant overexpression in Escherichia coli strains Rosetta 2, T7 Express and T7 Express (pGro7) and Pichia pastoris strain X-33, with a much higher expression level and 4800fold higher enzyme activity in Pichia pastoris, fed-batch cultivation of a Pichia pastoris transformant, method evaluation, overview | Colletotrichum gloeosporioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | D-xylose | recombinant enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
10.1 | - |
D-glucose | recombinant enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
10.2 | - |
D-glucose | native enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
17.1 | - |
D-glucose | recombinant enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
19 | - |
D-glucose | native enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
21 | - |
D-xylose | native enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
24 | - |
D-xylose | native enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
26 | - |
D-xylose | recombinant enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, deglycosylated recombinant enzyme expressed from Pichia pastoris, SDS-PAGE | Colletotrichum gloeosporioides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + a quinone | Colletotrichum gloeosporioides | - |
D-glucono-1,5-lactone + a quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colletotrichum gloeosporioides | - |
anamorph Colletotrichum gloeosporoides | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Colletotrichum gloeosporioides |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris strain X-33 5.1fold by hydrophobic interaction and anion exchange chromatography | Colletotrichum gloeosporioides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
836 | - |
purified recombinant enzyme expressed from Pichia pastoris, pH 7.5, 30°C | Colletotrichum gloeosporioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + a quinone | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + a quinol | - |
? | |
D-glucose + ferricenium ion | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + ferrocenium ion | - |
? | |
D-xylose + ferricenium ion | - |
Colletotrichum gloeosporioides | D-xylono-1,5-lactone + ferrocenium ion | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, deglycosylated recombinant enzyme expressed from Pichia pastoris, SDS-PAGE | Colletotrichum gloeosporioides |
Synonyms | Comment | Organism |
---|---|---|
D-glucose:acceptor 1-oxidoreductase | - |
Colletotrichum gloeosporioides |
FAD-dependent glucose dehydrogenase | - |
Colletotrichum gloeosporioides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
recombinant enzyme expressed from Pichia pastoris | Colletotrichum gloeosporioides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
pH-dependent thermal stability with the highest Tm values in the acidic range of pH 4.5 to pH 6.4. The maximum Tm value of 56°C is measured in 50 mM sodium acetate buffer pH 5.0 and in 50 mM MES buffer pH 5.8 | Colletotrichum gloeosporioides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
D-xylose | native enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
53 | - |
D-xylose | recombinant enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
60 | - |
D-xylose | native enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
61 | - |
D-xylose | recombinant enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
179 | - |
D-glucose | recombinant enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
180 | - |
D-glucose | native enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
380 | - |
D-glucose | native enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
418 | - |
D-glucose | recombinant enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant enzyme | Colletotrichum gloeosporioides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 5.8 | pH-dependent thermal stability with the highest Tm values in the acidic range of pH 4.5 to pH 6.4. The maximum Tm value of 56°C is measured in 50 mM sodium acetate buffer pH 5.0 and in 50 mM MES buffer pH 5.8 | Colletotrichum gloeosporioides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Colletotrichum gloeosporioides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
D-xylose | native enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
2 | - |
D-xylose | recombinant enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
2.5 | - |
D-xylose | native enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
2.7 | - |
D-xylose | recombinant enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
17.6 | - |
D-glucose | native enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
17.7 | - |
D-glucose | recombinant enzyme, pH 5.5, 30°C | Colletotrichum gloeosporioides | |
20 | - |
D-glucose | native enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides | |
24.5 | - |
D-glucose | recombinant enzyme, pH 7.5, 30°C | Colletotrichum gloeosporioides |