Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | urea causes a dose-dependent but reversible inhibition by dissoziation of the hetero-oligomeric enzyme comparable with the effect of heat-treatment, 3.5 M urea reduces GDH activity by 20%, 5 M urea by 80% and 8 M urea by ca. 90% | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
D-glucose | at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia | |
1.4 | - |
phenazine methosulfate | at 40°C | Burkholderia cepacia | |
2.5 | - |
D-glucose | at 40°C | Burkholderia cepacia | |
2.7 | - |
phenazine methosulfate | at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
67000 | - |
SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring | - |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
290 micromol/min/mg Vmax of oxidation of glucose at 40°C | Burkholderia cepacia |
additional information | - |
4.3 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
additional information | - |
470 micromol/min/mg Vmax of oxidation of phenazine methosulfate at 40°C | Burkholderia cepacia |
additional information | - |
66 micromol/min/mg Vmax of oxidation of glucose at 40°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + phenazine methosulfate | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heteromer | SDS-PAGE, x * 67000, alpha-subunit, x * 43000, beta subunit, determined as cytochrome c via spectroscopic analyses | Burkholderia cepacia |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Burkholderia cepacia |
glucose dehydrogenase | - |
Burkholderia cepacia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
after preincubation with 5.8 M urea for 30 min at 25°C | Burkholderia cepacia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | after preincubation with 5.8 M urea for 30 min at 25°C | Burkholderia cepacia |