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Literature summary for 1.1.5.9 extracted from
- Studies on glucose dehydrogenase of Aspergillus oryzae. II. Purification and physical and chemical properties (1967), Biochim. Biophys. Acta, 139, 277-293.
General Stability
| General Stability | Organism |
|---|---|
| glucose, 0.2 M, protects during heat treatment | Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 118000 | - |
low speed sedimentation without reaching equilibrium | Aspergillus oryzae |
| 120000 | - |
gel filtration | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 24% carbohydrate consisting of glucose, mannose and hexosamines | Aspergillus oryzae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Aspergillus oryzae | - |
| mycelium | - |
Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus oryzae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, 0.05 M potassium phosphate buffer, pH 6.5, 10% loss of activity after 3 months, 50% loss of activity after 6 months | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + 2,6-dichlorophenolindophenol | - |
Aspergillus oryzae | D-gluconic acid + reduced 2,6-dichlorophenolindophenol | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
15 min, stable | Aspergillus oryzae |
| 50 | - |
15 min, about 40% loss of activity | Aspergillus oryzae |
| 60 | - |
15 min, complete loss of activity | Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
30°C, 2 h, about 40% loss of activity | Aspergillus oryzae |
| 6 | 8 | 30°C, 2 h, stable, rapid inactivation outside this range | Aspergillus oryzae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 1 mol per mol of enzyme | Aspergillus oryzae | |
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Release 2023.1 (January 2023)
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