BRENDA - Enzyme Database
show all sequences of 1.1.5.4

Cyanide degradation by Pseudomonas pseudoalcaligenes CECT5344 involves a malate:quinone oxidoreductase and an associated cyanide-insensitive electron transfer chain

Luque-Almagro, V.M.; Merchan, F.; Blasco, R.; Igeno, M.I.; Martinez-Luque, M.; Moreno-Vivian, C.; Castillo, F.; Roldan, M.D.; Microbiology 157, 739-746 (2011)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas pseudoalcaligenes
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-malate + ubiquinone
Pseudomonas pseudoalcaligenes
-
oxaloacetate + ubiquinol
-
-
?
(S)-malate + ubiquinone
Pseudomonas pseudoalcaligenes CECT 5344
-
oxaloacetate + ubiquinol
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas pseudoalcaligenes
-
isolated from the Guadalquivir River
-
Pseudomonas pseudoalcaligenes CECT 5344
-
isolated from the Guadalquivir River
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-malate + ubiquinone
-
723124
Pseudomonas pseudoalcaligenes
oxaloacetate + ubiquinol
-
-
-
?
(S)-malate + ubiquinone
with dichlorophenolindophenol as terminal acceptor
723124
Pseudomonas pseudoalcaligenes
oxaloacetate + ubiquinol
-
-
-
?
(S)-malate + ubiquinone
-
723124
Pseudomonas pseudoalcaligenes CECT 5344
oxaloacetate + ubiquinol
-
-
-
?
(S)-malate + ubiquinone
with dichlorophenolindophenol as terminal acceptor
723124
Pseudomonas pseudoalcaligenes CECT 5344
oxaloacetate + ubiquinol
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
malate: quinone oxidoreductase
-
Pseudomonas pseudoalcaligenes
malate:quinone oxidoreductase
-
Pseudomonas pseudoalcaligenes
Mqo
-
Pseudomonas pseudoalcaligenes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas pseudoalcaligenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas pseudoalcaligenes
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is active with 2,6-dichlorophenolindophenol
Pseudomonas pseudoalcaligenes
ubiquinone
-
Pseudomonas pseudoalcaligenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is active with 2,6-dichlorophenolindophenol
Pseudomonas pseudoalcaligenes
ubiquinone
-
Pseudomonas pseudoalcaligenes
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Pseudomonas pseudoalcaligenes
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-malate + ubiquinone
Pseudomonas pseudoalcaligenes
-
oxaloacetate + ubiquinol
-
-
?
(S)-malate + ubiquinone
Pseudomonas pseudoalcaligenes CECT 5344
-
oxaloacetate + ubiquinol
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-malate + ubiquinone
-
723124
Pseudomonas pseudoalcaligenes
oxaloacetate + ubiquinol
-
-
-
?
(S)-malate + ubiquinone
with dichlorophenolindophenol as terminal acceptor
723124
Pseudomonas pseudoalcaligenes
oxaloacetate + ubiquinol
-
-
-
?
(S)-malate + ubiquinone
-
723124
Pseudomonas pseudoalcaligenes CECT 5344
oxaloacetate + ubiquinol
-
-
-
?
(S)-malate + ubiquinone
with dichlorophenolindophenol as terminal acceptor
723124
Pseudomonas pseudoalcaligenes CECT 5344
oxaloacetate + ubiquinol
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Pseudomonas pseudoalcaligenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas pseudoalcaligenes
General Information
General Information
Commentary
Organism
additional information
oxaloacetate reacts chemically inside the cyanide-insensitive cells to produce a cyanohydrin (2-hydroxynitrile), which is further converted to ammonium. The nitrile is transiently accumulated in cyanide-containing media
Pseudomonas pseudoalcaligenes
physiological function
the enzyme is a component of the electron transfer chain in Pseudomonas pseudoalcaligenes CECT5344 cells, overview
Pseudomonas pseudoalcaligenes
General Information (protein specific)
General Information
Commentary
Organism
additional information
oxaloacetate reacts chemically inside the cyanide-insensitive cells to produce a cyanohydrin (2-hydroxynitrile), which is further converted to ammonium. The nitrile is transiently accumulated in cyanide-containing media
Pseudomonas pseudoalcaligenes
physiological function
the enzyme is a component of the electron transfer chain in Pseudomonas pseudoalcaligenes CECT5344 cells, overview
Pseudomonas pseudoalcaligenes
Other publictions for EC 1.1.5.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722575
Kabashima
Purification and characterizat ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) PS3
J. Bioenerg. Biomembr.
45
131-136
2013
-
-
1
-
-
-
8
-
-
-
2
2
-
17
-
-
-
-
-
-
-
-
11
1
3
1
-
-
-
1
-
-
4
-
-
-
-
-
1
4
-
-
-
-
8
-
-
-
-
2
2
-
-
-
-
-
-
-
-
11
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
721563
Igeno
Metabolic adaptation of Pseudo ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes CECT 5344
Biochem. Soc. Trans.
39
1849-1853
2011
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723124
Luque-Almagro
Cyanide degradation by Pseudom ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes CECT 5344
Microbiology
157
739-746
2011
-
-
-
-
-
-
-
-
1
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
3
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
698612
Mellgren
Mqo, a tricarboxylic acid cycl ...
Pseudomonas syringae
J. Bacteriol.
191
3132-3141
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698670
Mogi
Polymyxin B identified as an i ...
Mycolicibacterium smegmatis
J. Biochem.
146
491-499
2009
-
-
-
-
-
-
3
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
3
1
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
1
3
1
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687185
Fleige
Localisation of gluconeogenesi ...
Toxoplasma gondii
Int. J. Parasitol.
38
1121-1132
2008
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679906
Diaz-Perez
Identification of the aceA gen ...
Pseudomonas aeruginosa
FEMS Microbiol. Lett.
269
309-316
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696805
Mitsuhashi
Disruption of malate:quinone o ...
Corynebacterium glutamicum
Biosci. Biotechnol. Biochem.
70
2803-2806
2006
-
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673714
Foerster-Fromme
Malate:quinone oxidoreductase ...
Pseudomonas aeruginosa, Pseudomonas citronellolis
FEMS Microbiol. Lett.
246
25-31
2005
-
-
2
-
2
-
-
-
2
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
6
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
287737
Kather
Another unusual type of citric ...
Helicobacter pylori
J. Bacteriol.
182
3204-3209
2000
-
-
1
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287738
Molenaar
Functions of the membrane-asso ...
Corynebacterium glutamicum
J. Bacteriol.
182
6884-6891
2000
-
-
-
-
-
-
-
-
3
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698558
van der Rest
Functions of the membrane-asso ...
Escherichia coli
J. Bacteriol.
182
6892-6899
2000
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287733
Molenaar
Biochemical and genetic charac ...
Corynebacterium glutamicum
Eur. J. Biochem.
254
395-403
1998
6
-
1
-
-
-
1
-
2
-
-
1
-
6
-
-
1
-
-
-
-
2
3
-
3
-
-
-
-
-
-
-
1
-
-
-
6
-
1
1
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287734
Imai
FAD-dependent malate dehydroge ...
Mycobacterium sp., Mycobacterium sp. Takeo
Biochim. Biophys. Acta
523
37-46
1978
2
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
1
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
287730
Imai
-
A phospholipid-requiring enzym ...
Mycolicibacterium phlei
J. Biol. Chem.
248
7487-7494
1973
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
3
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287619
Phizackerley
-
Malate dehydrogenase (FAD-link ...
Pseudomonas putida, Pseudomonas putida Chester
Methods Enzymol.
13
135-140
1969
1
-
-
-
-
-
1
3
1
-
-
-
-
4
-
-
1
-
-
-
1
2
11
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
1
-
3
1
-
-
-
-
-
-
1
-
-
1
2
11
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
696072
Phizackerley
Cofactor requirements of the L ...
Pseudomonas putida, Pseudomonas putida Chester
Biochem. J.
101
524-535
1966
1
-
-
-
-
-
-
2
1
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
1
-
-
-
1
-
-
5
-
-
-
1
-
-
5
-
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-