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Literature summary for 1.1.5.4 extracted from
- Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum (1998), Eur. J. Biochem., 254, 395-403.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-methyl-1,4-naphthoquinone | reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 | Corynebacterium glutamicum |  |
| decylubiquinone | reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 | Corynebacterium glutamicum | |
| duroquinone | reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 | Corynebacterium glutamicum | |
| Lipid | activates | Corynebacterium glutamicum | |
| ubiquinone-0 | reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 | Corynebacterium glutamicum | |
| ubiquinone-1 | reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 | Corynebacterium glutamicum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Corynebacterium glutamicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KSCN | - |
Corynebacterium glutamicum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | a peripheral membrane protein that can be released from the membrane by addition of chelators | Corynebacterium glutamicum | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + acceptor | Corynebacterium glutamicum | the enzyme takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. A mutant completely lacking Mqo activity grows poorly on several substrates tested. This enzyme might be especially important when a net flux from malate to oxaloacetate is required, but the intracellular concentrations of the reactants are unfavourable for the NAD-dependent reaction (EC 1.1.1.37) | oxaloacetate + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | O69282 | a mutant completely lacking Mqo activity grows poorly on several substrates tested | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and His-tagged enzyme | Corynebacterium glutamicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| when frozen, the activity is stable for several months | Corynebacterium glutamicum |
| when stored on ice, the half-life is approximately 120 h, important stabilizing conditions for storage on ice are the presence of EDTA and EGTA. the presence of glycerol, and pH 6. The presence of Mg2+ and Ca2+ has a destabilizing effect | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + 2,6-dichlorophenol indophenol | - |
Corynebacterium glutamicum | oxaloacetate + reduced 2,6-dichlorophenol indophenol | - |
? | |
| (S)-malate + acceptor | the enzyme takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. A mutant completely lacking Mqo activity grows poorly on several substrates tested. This enzyme might be especially important when a net flux from malate to oxaloacetate is required, but the intracellular concentrations of the reactants are unfavourable for the NAD-dependent reaction (EC 1.1.1.37) | Corynebacterium glutamicum | oxaloacetate + reduced acceptor | - |
? | |
| (S)-malate + ubiquinone-1 | ubiquinone-1 is directly reduced by the enzyme | Corynebacterium glutamicum | oxaloacetate + reduced ubiquinone-1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malate dehydrogenase (acceptor) | - |
Corynebacterium glutamicum |
| malate:quinone oxidoreductase | - |
Corynebacterium glutamicum |
| Mqo | - |
Corynebacterium glutamicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | is probably a tightly but non-covalently bound prosthetic group | Corynebacterium glutamicum | |
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