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Literature summary for 1.1.5.3 extracted from

  • Lalle, M.; Camerini, S.; Cecchetti, S.; Finelli, R.; Sferra, G.; Mueller, J.; Ricci, G.; Pozio, E.
    The FAD-dependent glycerol-3-phosphate dehydrogenase of Giardia duodenalis: an unconventional enzyme that interacts with the g14-3-3 and it is a target of the antitumoral compound NBDHEX (2015), Front. Microbiol., 6, 544.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.5.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol
-
 Giardia intestinalis
metronidazole
-
 Giardia intestinalis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Giardia intestinalis 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
119000
-
-
 Giardia intestinalis
120000
-
 x * 120000, SDS-PAGE Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + a quinone Giardia intestinalis
-
 glycerone phosphate + a quinol
-
 ?
sn-glycerol 3-phosphate + a quinone Giardia intestinalis WB-C6
-
 glycerone phosphate + a quinol
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-
Giardia intestinalis WB-C6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel resin column chromatography Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + a quinone
-
 Giardia intestinalis glycerone phosphate + a quinol
-
 ?
sn-glycerol 3-phosphate + a quinone
-
 Giardia intestinalis WB-C6 glycerone phosphate + a quinol
-
 ?
sn-glycerol 3-phosphate + phenazine methosulfate
-
 Giardia intestinalis ?
-
 ?
sn-glycerol 3-phosphate + phenazine methosulfate
-
 Giardia intestinalis WB-C6 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 120000, SDS-PAGE Giardia intestinalis
? x * 119000, calculated from amino acid sequence Giardia intestinalis

Synonyms

Synonyms Comment Organism
FAD-dependent glycerol-3-phosphate dehydrogenase
-
 Giardia intestinalis
flavin adenine dinucleotide-dependent glycerol-3-phosphate dehydrogenase
-
 Giardia intestinalis
G3PD
-
 Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Giardia intestinalis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0006
-
 at pH 7.5 and 25°C Giardia intestinalis 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol
0.0019
-
 at pH 7.5 and 25°C Giardia intestinalis metronidazole

General Information

General Information Comment Organism
physiological function the enzyme is involved in glycolysis, electron transport, glycerophospholipids metabolism, and hyperosmotic stress response Giardia intestinalis