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Literature summary for 1.1.5.3 extracted from
- Chemical and functional properties of the native and reconstituted forms of the membrane-bound, aerobic glycerol-3-phosphate dehydrogenase of Escherichia coli (1978), J. Biol. Chem., 253, 783-788.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| detergent | detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity | Escherichia coli | |
| Phospholipid | detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Escherichia coli | |
| D-2-Phosphoglyceric acid | - |
Escherichia coli | |
| D-3-phosphoglyceric acid | - |
Escherichia coli | |
| dihydroxyacetone phosphate | - |
Escherichia coli | |
| NEM | - |
Escherichia coli | |
| PCMB | - |
Escherichia coli | |
| phosphoenolpyruvate | - |
Escherichia coli | |
| Phosphoglycolic acid | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
2 * 58000, SDS-PAGE | Escherichia coli |
| 130000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol | - |
Escherichia coli | glycerone phosphate + reduced 2,6-dichlorophenylindophenol | - |
? | |
| sn-glycerol 3-phosphate + ferricyanide | - |
Escherichia coli | glycerone phosphate + ferrocyanide | - |
? | |
| sn-glycerol 3-phosphate + menadione | - |
Escherichia coli | glycerone phosphate + reduced menadione | - |
? | |
| sn-glycerol 3-phosphate + methylene blue | - |
Escherichia coli | glycerone phosphate + reduced methylene blue | - |
? | |
| sn-glycerol 3-phosphate + phenazine methosulfate | - |
Escherichia coli | glycerone phosphate + reduced phenazine methosulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | - |
- |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 0.4 mol of FAD per protein monomer of 58000 Da | Escherichia coli | |
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