Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | stimulates the reaction with phenazine methosulfate and methylene blue. 20% increase in activity of the reaction with phenazine methosulfate with a half-maximal concentration of 200 nM | Escherichia coli | |
FMN | stimulates the reaction with phenazine methosulfate. 6fold increase in activity with a half-maximal concentration of 0.13 mM | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Escherichia coli | |
DTNB | - |
Escherichia coli | |
FMN | reaction with methylene blue | Escherichia coli | |
PCMB | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
glycerol 3-phosphate | in absence of exogenous FMN | Escherichia coli | |
0.339 | - |
glycerol 3-phosphate | in presence of exogenous FMN | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated with membrane | Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 2 mol of non-heme iron per dimer | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
1 * 43000 + 1 * 62000, SDS-PAGE | Escherichia coli |
62000 | - |
1 * 43000 + 1 * 62000, SDS-PAGE | Escherichia coli |
93500 | - |
equilibrium sedimentation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C or 4°C, concentrated to 4-10 mg/ml, stable | Escherichia coli |
4°C, pH 6.0-8.5, 20% ethylene glycol v/v, 10% loss of activity after 60 h | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + methylene blue | - |
Escherichia coli | glycerone phosphate + reduced methylene blue | - |
? | |
sn-glycerol 3-phosphate + phenazine methosulfate | - |
Escherichia coli | glycerone phosphate + reduced phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 43000 + 1 * 62000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.3 | - |
glycerol 3-phosphate | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1 mol of noncovalently bound FAD per dimer | Escherichia coli |