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Literature summary for 1.1.5.2 extracted from
- Enhancement of lactobionic acid productivity by homologous expression of quinoprotein glucose dehydrogenase in Pseudomonas taetrolens (2020), J. Agric. Food Chem., 68, 12336-12344 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GDH1 gene is homologously expressed in Pseudomonas taetrolens or heterologously expressed in Escherichia coli. Recombinant expression of the GDH1 gene enhances intracellular lactose oxidizing activity and lactobionic acid production of Pseudomonas taetrolens in flask culture | Pseudomonas taetrolens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
D-glucose | pH 5.5, 30°C | Pseudomonas taetrolens |  |
| 0.46 | - |
maltose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.54 | - |
cellobiose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.6 | - |
D-galactose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.63 | - |
lactose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.67 | - |
D-xylose | pH 5.5, 30°C | Pseudomonas taetrolens | |
| 0.83 | - |
sucrose | pH 5.5, 30°C | Pseudomonas taetrolens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound, enzyme GDH1 | Pseudomonas taetrolens | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas taetrolens | A0A0J6JEN3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + 2,6-dichlorophenolindophenol | 58% of the activity as compared to D-glucose | Pseudomonas taetrolens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-galactose + 2,6-dichlorophenolindophenol | 48% of the activity as compared to D-glucose | Pseudomonas taetrolens | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-glucose + 2,6-dichlorophenolindophenol | - |
Pseudomonas taetrolens | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-xylose + 2,6-dichlorophenolindophenol | 32% of the activity as compared to D-glucose | Pseudomonas taetrolens | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| lactose + 2,6-dichlorophenolindophenol | 40% of the activity as compared to D-glucose | Pseudomonas taetrolens | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + 2,6-dichlorophenolindophenol | 91% of the activity as compared to D-glucose | Pseudomonas taetrolens | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| sucrose + 2,6-dichlorophenolindophenol | 30% of the activity as compared to D-glucose | Pseudomonas taetrolens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH1 | - |
Pseudomonas taetrolens |
| quinoprotein glucose dehydrogenase | - |
Pseudomonas taetrolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas taetrolens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Pseudomonas taetrolens |
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