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Literature summary for 1.1.5.2 extracted from

  • Kim, H.; Wang, J.; Lee, J.; Park, A.; Park, H.; Jeon, S.
    Biochemical and structural characterization of quinoprotein aldose sugar dehydrogenase from Thermus thermophilus HJ6 Mutational analysis of Tyr156 in the substrate-binding site (2016), Arch. Biochem. Biophys., 608, 20-26 .
    View publication on PubMed

Application

Application Comment Organism
analysis its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells Thermus thermophilus
biofuel production its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21, wild-type and mutant enzymes Y156A and Y156K Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
Y156A impaired activities and affinities for all substrates and completely loses the activity for alcohols. The catalytic efficiency of the Y156A mutant is lower than that of the Y156K mutant for most substrates Thermus thermophilus
Y156K impaired activities and affinities for all substrates and completely loses the activity for alcohols. The catalytic efficiency of the Y156A mutant is lower than that of the Y156K mutant for most substrates Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000004
-
2-deoxy-D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00002
-
D-glucosamine pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00002
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00002
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00007
-
D-cellobiose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00007
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00008
-
D-glucose 6-phosphate pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00008
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00009
-
xylitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00009
-
mannitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00011
-
D-galactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00013
-
maltotriose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00013
-
D-mannose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00013
-
D-ribose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00014
-
D-xylose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00014
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00014
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00014
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00015
-
methanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00016
-
L-arabinose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00016
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00016
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00017
-
maltose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00017
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00018
-
ethanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00019
-
alpha-D-lactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.0002
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00021
-
D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.00023
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00023
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00025
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00026
-
maltose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.0003
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00037
-
maltose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00038
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00044
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.00046
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00047
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.0006
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00064
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.00065
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
SDS-PAGE Thermus thermophilus
38871
-
calculated from sequence Thermus thermophilus
80000
-
gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus I7A144
-
-
Thermus thermophilus HJ6 I7A144
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose + phenazine methosulfate
-
Thermus thermophilus 2-deoxy-D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
2-deoxy-D-glucose + phenazine methosulfate
-
Thermus thermophilus HJ6 2-deoxy-D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
alpha-D-lactose + phenazine methosulfate
-
Thermus thermophilus alpha-D-lactono-1,5-lactone + reduced phenazine methosulfate
-
?
alpha-D-lactose + phenazine methosulfate
-
Thermus thermophilus HJ6 alpha-D-lactono-1,5-lactone + reduced phenazine methosulfate
-
?
D-cellobiose + phenazine methosulfate
-
Thermus thermophilus D-cellobiono-1,5-lactone + reduced phenazine methosulfate
-
?
D-galactose + phenazine methosulfate
-
Thermus thermophilus D-galactono-1,5-lactone + reduced phenazine methosulfate
-
?
D-glucosamino-1,5-lactone + reduced phenazine methosulfate
-
Thermus thermophilus D-glucosamine + reduced phenazine methosulfate
-
?
D-glucose + phenazine methosulfate
-
Thermus thermophilus D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
D-glucose + phenazine methosulfate
-
Thermus thermophilus HJ6 D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
D-glucose 6-phosphate + phenazine methosulfate
-
Thermus thermophilus 6-phospho-D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
D-mannose + phenazine methosulfate
-
Thermus thermophilus D-mannono-1,5-lactone + reduced phenazine methosulfate
-
?
D-ribose + phenazine methosulfate
-
Thermus thermophilus D-ribono-1,4-lactone + reduced phenazine methosulfate
-
?
D-xylose + phenazine methosulfate
-
Thermus thermophilus D-xylono-1,5-lactone + reduced phenazine methosulfate
-
?
ethanol + phenazine methosulfate
-
Thermus thermophilus acetaldehyde + reduced phenazine methosulfate
-
?
ethanol + phenazine methosulfate
-
Thermus thermophilus HJ6 acetaldehyde + reduced phenazine methosulfate
-
?
L-arabinose + phenazine methosulfate
-
Thermus thermophilus D-arabinono-1,5-lactone + reduced phenazine methosulfate
-
?
maltose + phenazine methosulfate
-
Thermus thermophilus D-maltono-1,5-lactone + reduced phenazine methosulfate
-
?
maltotriose + phenazine methosulfate
-
Thermus thermophilus D-maltotriono-1,5-lactone + reduced phenazine methosulfate
-
?
mannitol + phenazine methosulfate
-
Thermus thermophilus ?
-
?
methanol + phenazine methosulfate
-
Thermus thermophilus formaldehyde + reduced phenazine methosulfate
-
?
methanol + phenazine methosulfate
-
Thermus thermophilus HJ6 formaldehyde + reduced phenazine methosulfate
-
?
xylitol + phenazine methosulfate
-
Thermus thermophilus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 37000, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
quinoprotein aldose sugar dehydrogenase
-
Thermus thermophilus
Tt_ASD
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
about 50% of maximal activity Thermus thermophilus
60 80 the recombinant enzyme shows more than 90% activity within the range of 60-80°C Thermus thermophilus
85
-
about 50% of maximal activity Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
half-life of apoenzyme: about 25 min. Activity of the pyrroloquinoline quinone-bound holoenzyme is not lost after incubation at 85°C for 100 min Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15
-
xylitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
38
-
mannitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
69
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
76
-
methanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
104
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
117
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
123
-
ethanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
128
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
142
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
154
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
160
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
160
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
170
-
maltose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
181
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
186
-
maltose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
186
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
192
-
D-glucose 6-phosphate pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
193
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
197
-
D-glucosamine pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
204
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
225
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
260
-
D-ribose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
261
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
265
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
282
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
305
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
316
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
339
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
363
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
715
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
772
-
maltotriose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
822
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
823
-
D-galactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1022
-
maltose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1042
-
D-cellobiose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1054
-
D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1141
-
2-deoxy-D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1627
-
D-mannose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1681
-
D-xylose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1755
-
L-arabinose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
1953
-
alpha-D-lactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Thermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone prosthetic group Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.173
-
xylitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.343
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.414
-
mannitol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.417
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.439
-
alpha-D-lactose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.5
-
maltose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.506
-
methanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.527
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.654
-
maltose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.68
-
ethanol pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
0.699
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.719
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.796
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.808
-
D-cellobiose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.872
-
D-xylose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.903
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
0.921
-
2-deoxy-D-glucose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
0.952
-
D-galactose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.079
-
L-arabinose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.287
-
D-glucose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.366
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
1.61
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.631
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
1.858
-
D-mannose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
2.062
-
D-ribose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
2.283
-
maltotriose pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
2.543
-
D-glucose 6-phosphate pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
2.724
-
D-ribose pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
5.021
-
D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
5.727
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156A Thermus thermophilus
6.012
-
maltose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
6.16
-
maltotriose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
7.488
-
D-galactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
8.696
-
D-glucosamine pH 7.5, 70°C, mutant enzyme Y156K Thermus thermophilus
10.28
-
alpha-D-lactose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
10.972
-
L-arabinose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
12.011
-
D-xylose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
12.518
-
D-mannose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
12.989
-
D-glucosamine pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
14.893
-
D-cellobiose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus
29.483
-
2-deoxy-D-glucose pH 7.5, 70°C, wild-type enzyme Thermus thermophilus