Application | Comment | Organism |
---|---|---|
analysis | its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells | Thermus thermophilus |
biofuel production | its high stability at high temperature makes this enzyme potentially useful for applications in biosensors or biofuel cells | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21, wild-type and mutant enzymes Y156A and Y156K | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
Y156A | impaired activities and affinities for all substrates and completely loses the activity for alcohols. The catalytic efficiency of the Y156A mutant is lower than that of the Y156K mutant for most substrates | Thermus thermophilus |
Y156K | impaired activities and affinities for all substrates and completely loses the activity for alcohols. The catalytic efficiency of the Y156A mutant is lower than that of the Y156K mutant for most substrates | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00002 | - |
D-glucosamine | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00002 | - |
D-glucosamine | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00002 | - |
D-glucosamine | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00007 | - |
D-cellobiose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00007 | - |
D-ribose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00008 | - |
D-glucose 6-phosphate | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00008 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00009 | - |
xylitol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00009 | - |
mannitol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00011 | - |
D-galactose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00013 | - |
maltotriose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00013 | - |
D-mannose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00013 | - |
D-ribose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00014 | - |
D-xylose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00014 | - |
D-mannose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00014 | - |
L-arabinose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00014 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00015 | - |
methanol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00016 | - |
L-arabinose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00016 | - |
maltotriose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00016 | - |
D-mannose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00017 | - |
maltose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00017 | - |
L-arabinose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00018 | - |
ethanol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00019 | - |
alpha-D-lactose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.0002 | - |
D-galactose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00021 | - |
D-glucose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.00023 | - |
D-xylose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00023 | - |
D-cellobiose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00025 | - |
D-glucose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00026 | - |
maltose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.0003 | - |
D-cellobiose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00037 | - |
maltose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00038 | - |
D-galactose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00044 | - |
D-ribose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.00046 | - |
D-xylose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00047 | - |
D-glucose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.0006 | - |
maltotriose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00064 | - |
alpha-D-lactose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.00065 | - |
alpha-D-lactose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
SDS-PAGE | Thermus thermophilus |
38871 | - |
calculated from sequence | Thermus thermophilus |
80000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | I7A144 | - |
- |
Thermus thermophilus HJ6 | I7A144 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + phenazine methosulfate | - |
Thermus thermophilus | 2-deoxy-D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
2-deoxy-D-glucose + phenazine methosulfate | - |
Thermus thermophilus HJ6 | 2-deoxy-D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
alpha-D-lactose + phenazine methosulfate | - |
Thermus thermophilus | alpha-D-lactono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
alpha-D-lactose + phenazine methosulfate | - |
Thermus thermophilus HJ6 | alpha-D-lactono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-cellobiose + phenazine methosulfate | - |
Thermus thermophilus | D-cellobiono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-galactose + phenazine methosulfate | - |
Thermus thermophilus | D-galactono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-glucosamino-1,5-lactone + reduced phenazine methosulfate | - |
Thermus thermophilus | D-glucosamine + reduced phenazine methosulfate | - |
? | |
D-glucose + phenazine methosulfate | - |
Thermus thermophilus | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-glucose + phenazine methosulfate | - |
Thermus thermophilus HJ6 | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-glucose 6-phosphate + phenazine methosulfate | - |
Thermus thermophilus | 6-phospho-D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-mannose + phenazine methosulfate | - |
Thermus thermophilus | D-mannono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-ribose + phenazine methosulfate | - |
Thermus thermophilus | D-ribono-1,4-lactone + reduced phenazine methosulfate | - |
? | |
D-xylose + phenazine methosulfate | - |
Thermus thermophilus | D-xylono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
ethanol + phenazine methosulfate | - |
Thermus thermophilus | acetaldehyde + reduced phenazine methosulfate | - |
? | |
ethanol + phenazine methosulfate | - |
Thermus thermophilus HJ6 | acetaldehyde + reduced phenazine methosulfate | - |
? | |
L-arabinose + phenazine methosulfate | - |
Thermus thermophilus | D-arabinono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
maltose + phenazine methosulfate | - |
Thermus thermophilus | D-maltono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
maltotriose + phenazine methosulfate | - |
Thermus thermophilus | D-maltotriono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
mannitol + phenazine methosulfate | - |
Thermus thermophilus | ? | - |
? | |
methanol + phenazine methosulfate | - |
Thermus thermophilus | formaldehyde + reduced phenazine methosulfate | - |
? | |
methanol + phenazine methosulfate | - |
Thermus thermophilus HJ6 | formaldehyde + reduced phenazine methosulfate | - |
? | |
xylitol + phenazine methosulfate | - |
Thermus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37000, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
quinoprotein aldose sugar dehydrogenase | - |
Thermus thermophilus |
Tt_ASD | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
about 50% of maximal activity | Thermus thermophilus |
60 | 80 | the recombinant enzyme shows more than 90% activity within the range of 60-80°C | Thermus thermophilus |
85 | - |
about 50% of maximal activity | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
half-life of apoenzyme: about 25 min. Activity of the pyrroloquinoline quinone-bound holoenzyme is not lost after incubation at 85°C for 100 min | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
xylitol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
38 | - |
mannitol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
69 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
76 | - |
methanol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
104 | - |
D-glucosamine | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
117 | - |
D-glucosamine | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
123 | - |
ethanol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
128 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
142 | - |
L-arabinose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
154 | - |
L-arabinose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
160 | - |
D-galactose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
160 | - |
D-cellobiose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
170 | - |
maltose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
181 | - |
D-ribose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
186 | - |
maltose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
186 | - |
D-cellobiose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
192 | - |
D-glucose 6-phosphate | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
193 | - |
D-galactose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
197 | - |
D-glucosamine | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
204 | - |
D-xylose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
225 | - |
alpha-D-lactose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
260 | - |
D-ribose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
261 | - |
D-mannose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
265 | - |
D-mannose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
282 | - |
alpha-D-lactose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
305 | - |
D-xylose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
316 | - |
D-glucose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
339 | - |
D-glucose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
363 | - |
maltotriose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
715 | - |
D-ribose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
772 | - |
maltotriose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
822 | - |
maltotriose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
823 | - |
D-galactose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1022 | - |
maltose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1042 | - |
D-cellobiose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1054 | - |
D-glucose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1141 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1627 | - |
D-mannose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1681 | - |
D-xylose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1755 | - |
L-arabinose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
1953 | - |
alpha-D-lactose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | prosthetic group | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.173 | - |
xylitol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.343 | - |
alpha-D-lactose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.414 | - |
mannitol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.417 | - |
D-galactose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.439 | - |
alpha-D-lactose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.5 | - |
maltose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.506 | - |
methanol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.527 | - |
D-cellobiose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.654 | - |
maltose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.68 | - |
ethanol | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
0.699 | - |
D-xylose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.719 | - |
D-glucose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.796 | - |
L-arabinose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.808 | - |
D-cellobiose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.872 | - |
D-xylose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.903 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
0.921 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
0.952 | - |
D-galactose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
1.079 | - |
L-arabinose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
1.287 | - |
D-glucose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
1.366 | - |
maltotriose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
1.61 | - |
D-mannose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
1.631 | - |
D-ribose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
1.858 | - |
D-mannose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
2.062 | - |
D-ribose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
2.283 | - |
maltotriose | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
2.543 | - |
D-glucose 6-phosphate | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
2.724 | - |
D-ribose | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
5.021 | - |
D-glucose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
5.727 | - |
D-glucosamine | pH 7.5, 70°C, mutant enzyme Y156A | Thermus thermophilus | |
6.012 | - |
maltose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
6.16 | - |
maltotriose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
7.488 | - |
D-galactose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
8.696 | - |
D-glucosamine | pH 7.5, 70°C, mutant enzyme Y156K | Thermus thermophilus | |
10.28 | - |
alpha-D-lactose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
10.972 | - |
L-arabinose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
12.011 | - |
D-xylose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
12.518 | - |
D-mannose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
12.989 | - |
D-glucosamine | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
14.893 | - |
D-cellobiose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus | |
29.483 | - |
2-deoxy-D-glucose | pH 7.5, 70°C, wild-type enzyme | Thermus thermophilus |