Literature summary for 1.1.5.2 extracted from

Durand, F.; Limoges, B.; Mano, N.; Mavre, F.; Miranda-Castro, R.; Saveant, J.M.
Effect of substrate inhibition and cooperativity on the electrochemical responses of glucose dehydrogenase. Kinetic characterization of wild and mutant types (2011), J. Am. Chem. Soc., 133, 12801-12809.

Search for more literature for 1.1.5.2

Application

Application	Comment	Organism
analysis	because of its high turnover number, PQQ-GDH is proposed as an enzyme label for the development of sensitive electrochemical enzyme-amplified bioaffinity assays. It has, for example, been applied to the amperometric detection of DNA hybrids or sandwich DNA aptamers at the surface of a carbon electrode	Acinetobacter calcoaceticus

Protein Variants

Protein Variants	Comment	Organism
N428C	site-directed mutagenesis, at relatively high concentrations of mediator and substrate, catalysis by the mutant type may be more efficient than with the wild-type	Acinetobacter calcoaceticus

Inhibitors

Inhibitors	Comment	Organism	Structure
D-glucose	substrate inhibition	Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	detailed kinetic analysis of wild-type and mutant enzymes, substrate inhibition and cooperativity effects, overview	Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	apo-GDH can be fully reconstituted in the dimeric holoform in the presence of pyrroloquinoline quinone and Ca2+	Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose + ubiquinone	Acinetobacter calcoaceticus	-	D-glucono-1,5-lactone + ubiquinol	-	?

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter calcoaceticus	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol	ping pong reaction mechanism	Acinetobacter calcoaceticus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose + ubiquinone	-	Acinetobacter calcoaceticus	D-glucono-1,5-lactone + ubiquinol	-	?
additional information	PQQ-GDH has a broad specificity toward the oxidation of aldose sugars (hexoses, pentoses, mono- and disaccharides) into the corresponding lactones and the reduction of artificial electron acceptors	Acinetobacter calcoaceticus	?	-	?

Subunits

Subunits	Comment	Organism
dimer	apo-GDH can be fully reconstituted in the dimeric holoform in the presence of pyrroloquinoline quinone and Ca2+	Acinetobacter calcoaceticus

Synonyms

Synonyms	Comment	Organism
PQQ-GDH	-	Acinetobacter calcoaceticus
quinoprotein glucose dehydrogenase	-	Acinetobacter calcoaceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1550	-	D-glucose	pH 7.0, temperature not specified in the publication, wild-type enzyme	Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Acinetobacter calcoaceticus

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	apo-GDH can be fully reconstituted in the dimeric holoform in the presence of pyrroloquinoline quinone and Ca2+	Acinetobacter calcoaceticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2100	-	D-glucose	pH 7.0, temperature not specified in the publication, wild-type enzyme	Acinetobacter calcoaceticus

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Release 2023.1 (January 2023)