Literature summary for 1.1.5.2 extracted from

Andreeva, I.G.; Golubeva, L.I.; Kuvaeva, T.M.; Gak, E.R.; Katashkina, J.I.; Mashko, S.V.
Identification of Pantoea ananatis gene encoding membrane pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and pqqABCDEF operon essential for PQQ biosynthesis (2011), FEMS Microbiol. Lett., 318, 55-60.

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Activating Compound

Activating Compound	Comment	Organism	Structure
pyrroloquinoline quinone	exogenously added cofector slightly activates the enzyme activity	Pantoea ananatis

Cloned(Commentary)

Cloned (Comment)	Organism
operon qqqABCDEF, gene gcd	Pantoea ananatis

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of DELTAgcd and DELTApqq mutants. qqqABCDEF is cloned in vivo and integrated into the chromosomes of Pantoea ananatis and Escherichia coli according to the Dual In/Out strategy. Introduction of a second copy of pqqABCDEF to Pantoea ananatis strain SC17(0) doubles the accumulation of PQQ. Integration of the operon into Escherichia coli strain MG1655DptsGDmanXY restores the growth of bacteria on glucose, overview	Pantoea ananatis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Pantoea ananatis	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose + ubiquinone	Pantoea ananatis	-	D-glucono-1,5-lactone + ubiquinol	-	?
D-glucose + ubiquinone	Pantoea ananatis SC17(0)	-	D-glucono-1,5-lactone + ubiquinol	-	?

Organism

Organism	UniProt	Comment	Textmining
Pantoea ananatis	D4P700	gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ	-
Pantoea ananatis SC17(0)	D4P700	gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00245	-	native enzyme in cells of strain SC17(0), pH 6.5, temperature not specified in the publication	Pantoea ananatis
0.0197	-	native enzyme in cells of strain SC17(0) in presence of 0.01 mM pyrroloquinoline quinone, pH 6.5, temperature not specified in the publication	Pantoea ananatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose + ubiquinone	-	Pantoea ananatis	D-glucono-1,5-lactone + ubiquinol	-	?
D-glucose + ubiquinone	assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol	Pantoea ananatis	D-glucono-1,5-lactone + ubiquinol	-	?
D-glucose + ubiquinone	-	Pantoea ananatis SC17(0)	D-glucono-1,5-lactone + ubiquinol	-	?
D-glucose + ubiquinone	assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol	Pantoea ananatis SC17(0)	D-glucono-1,5-lactone + ubiquinol	-	?

Synonyms

Synonyms	Comment	Organism
mGDH	-	Pantoea ananatis
PQQ-dependent glucose dehydrogenase	-	Pantoea ananatis
PQQ-dependent mGDH	-	Pantoea ananatis
quinone-dependent glucose dehydrogenase	-	Pantoea ananatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Pantoea ananatis

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	PQQ, essential cofactor for enzyme activity, essential role of pqqABCDEF in PQQ biosynthesis, overview	Pantoea ananatis

General Information

General Information	Comment	Organism
physiological function	essential role of pqqABCDEF in pyrroloquinoline quinone biosynthesis	Pantoea ananatis

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Release 2023.1 (January 2023)