Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | exogenously added cofector slightly activates the enzyme activity | Pantoea ananatis |
Cloned (Comment) | Organism |
---|---|
operon qqqABCDEF, gene gcd | Pantoea ananatis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of DELTAgcd and DELTApqq mutants. qqqABCDEF is cloned in vivo and integrated into the chromosomes of Pantoea ananatis and Escherichia coli according to the Dual In/Out strategy. Introduction of a second copy of pqqABCDEF to Pantoea ananatis strain SC17(0) doubles the accumulation of PQQ. Integration of the operon into Escherichia coli strain MG1655DptsGDmanXY restores the growth of bacteria on glucose, overview | Pantoea ananatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pantoea ananatis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | Pantoea ananatis | - |
D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | Pantoea ananatis SC17(0) | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea ananatis | D4P700 | gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ | - |
Pantoea ananatis SC17(0) | D4P700 | gene gcd, encoded in the operon pqqABCDEF together with cofactor pyrroloquinoline quinone, PQQ | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00245 | - |
native enzyme in cells of strain SC17(0), pH 6.5, temperature not specified in the publication | Pantoea ananatis |
0.0197 | - |
native enzyme in cells of strain SC17(0) in presence of 0.01 mM pyrroloquinoline quinone, pH 6.5, temperature not specified in the publication | Pantoea ananatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | - |
Pantoea ananatis | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Pantoea ananatis | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | - |
Pantoea ananatis SC17(0) | D-glucono-1,5-lactone + ubiquinol | - |
? | |
D-glucose + ubiquinone | assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Pantoea ananatis SC17(0) | D-glucono-1,5-lactone + ubiquinol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mGDH | - |
Pantoea ananatis |
PQQ-dependent glucose dehydrogenase | - |
Pantoea ananatis |
PQQ-dependent mGDH | - |
Pantoea ananatis |
quinone-dependent glucose dehydrogenase | - |
Pantoea ananatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Pantoea ananatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | PQQ, essential cofactor for enzyme activity, essential role of pqqABCDEF in PQQ biosynthesis, overview | Pantoea ananatis |
General Information | Comment | Organism |
---|---|---|
physiological function | essential role of pqqABCDEF in pyrroloquinoline quinone biosynthesis | Pantoea ananatis |