Literature summary for 1.1.5.2 extracted from

Sakuraba, H.; Yokono, K.; Yoneda, K.; Watanabe, A.; Asada, Y.; Satomura, T.; Yabutani, T.; Motonaka, J.; Ohshima, T.
Catalytic properties and crystal structure of quinoprotein aldose sugar dehydrogenase from hyperthermophilic archaeon Pyrobaculum aerophilum (2010), Arch. Biochem. Biophys., 502, 81-88.

Search for more literature for 1.1.5.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells	Pyrobaculum aerophilum

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoform and pyrroloquinoline quinone-bound holoenzyme, sitting drop vapor diffusion method, using 1.5 M ammonium sulfate and 0.1 M Tris/HCl buffer (pH 8.4), at 25°C	Pyrobaculum aerophilum

Inhibitors

Inhibitors	Comment	Organism	Structure
methylhydrazine	-	Pyrobaculum aerophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
390	-	D-galactose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
390	-	D-fructose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
500	-	D-xylose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
600	-	maltose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
680	-	D-glucose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
910	-	D-mannose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	gel filtration	Pyrobaculum aerophilum
32000	-	1 * 32000, SDS-PAGE	Pyrobaculum aerophilum
38452	-	1 * 38452, calculated from amino acid sequence	Pyrobaculum aerophilum
40000	-	dynamic light scattering	Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose + pyrroloquinoline quinone	Pyrobaculum aerophilum	-	D-glucono-1,5-lactone + pyrroloquinoline quinol	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum aerophilum	Q8ZUN8	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiPrep 16/10 SP XL column chromatography	Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.77	-	purified enzyme,in 50 mM BisTris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-cellobiose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	D-cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
D-fructose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	? + reduced 2,6-dichlorophenolindophenol	-	?
D-galactose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
D-glucose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
D-glucose + pyrroloquinoline quinone	-	Pyrobaculum aerophilum	D-glucono-1,5-lactone + pyrroloquinoline quinol	-	?
D-mannose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
D-ribose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	? + reduced 2,6-dichlorophenolindophenol	-	?
D-xylose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?
L-arabinose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	? + reduced 2,6-dichlorophenolindophenol	-	?
maltose + 2,6-dichloroindophenol	-	Pyrobaculum aerophilum	maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 32000, SDS-PAGE	Pyrobaculum aerophilum
monomer	1 * 38452, calculated from amino acid sequence	Pyrobaculum aerophilum

Synonyms

Synonyms	Comment	Organism
GDH	-	Pyrobaculum aerophilum
quinoprotein aldose sugar dehydrogenase	-	Pyrobaculum aerophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	above 75°C	Pyrobaculum aerophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
100	-	the enzyme is extremely thermostable, and the activity of the pyrroloquinoline quinone-bound holoenzyme is not lost after incubation at 100 °C for 10 min, while the apoenzyme retains full activity at up to 80°C under the same conditions	Pyrobaculum aerophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.3	-	maltose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
3.1	-	D-mannose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
3.2	-	D-xylose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
4.1	-	D-glucose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
4.3	-	D-galactose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	the optimum pH for D-glucose oxidation at 50°C is around pH 8.0	Pyrobaculum aerophilum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	11	when heated at 70°C for 30 min, the apo- and holoenzymes do not lose activity at pHs in the ranges of 4-11 and 3-11, respectively	Pyrobaculum aerophilum

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	-	Pyrobaculum aerophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
400	-	D-fructose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
1700	-	D-ribose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
2900	-	D-cellobiose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
3400	-	D-mannose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
3900	-	maltose	in 50 mM Bis-ris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
4100	-	L-arabinose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
6100	-	D-glucose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
6500	-	D-xylose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum
11000	-	D-galactose	in 50 mM Bis-Tris propane (pH 8.0), at 50°C	Pyrobaculum aerophilum

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Release 2023.1 (January 2023)