Application | Comment | Organism |
---|---|---|
biotechnology | enzyme has a great potential for application as glucose sensor constituent | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type isozyme PQQGDH-B and mutant enzymes in Escherichia coli | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
N340F/Y418F | site-directed mutagenesis, mutation of residues at the dimer interface, 2fold increased thermal stability at 55°C and unaltered catalytic efficiency compared to the wild-type enzyme | Acinetobacter calcoaceticus |
N340F/Y418I | site-directed mutagenesis, mutation of residues at the dimer interface, 2fold increased thermal stability at 55°C and unaltered catalytic efficiency compared to the wild-type enzyme | Acinetobacter calcoaceticus |
T416V/T417V | site-directed mutagenesis, mutation of resides of the hydrophobic region, 2fold increased thermal stability at 55°C and unaltered catalytic efficiency compared to the wild-type enzyme | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | predicted binding energy of wild-type and mutant enzymes | Acinetobacter calcoaceticus | |
16 | - |
D-glucose | recombinant mutant T416V/T417V, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
20 | - |
D-glucose | recombinant wild-type enzyme and mutants N340F/Y418F and N340F/Y418I, pH 7.0, 25°C | Acinetobacter calcoaceticus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | isozyme PQQGDH-B | Acinetobacter calcoaceticus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ubiquinone | Acinetobacter calcoaceticus | - |
D-glucono-1,5-lactone + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | P13650 | isozyme PQQGDH-B | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2500 | - |
mutant N340F/Y418I | Acinetobacter calcoaceticus |
2800 | - |
mutant T416V/T417V | Acinetobacter calcoaceticus |
3030 | - |
wild-type isozyme PQQGDH-B | Acinetobacter calcoaceticus |
3100 | - |
mutant N340F/Y418F | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | structure analysis, 6-blade beta-propeller protein with each blade consisting of a 4-stranded anti-parallel beta-sheet | Acinetobacter calcoaceticus |
More | the monomeric enzyme is not active, 3D models of wild-type and mutant enzymes | Acinetobacter calcoaceticus |
Synonyms | Comment | Organism |
---|---|---|
PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
PQQGDH-B | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter calcoaceticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
wild-type isozyme PQQGDH-B: half-life 9.5 min | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus |