Application | Comment | Organism |
---|---|---|
biotechnology | surface charge engineering of the enzyme for optimization of downstream processing in large scale enzyme production | Acinetobacter calcoaceticus |
Cloned (Comment) | Organism |
---|---|
expression in the periplasm of Escherichia coli strain PP2418, with and without a C-terminal tag of 3 Arg residues | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
Q209R/N240R/T389R | site-directed mutagenesis, increased thermal stability compared to the wild-type enzyme | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Acinetobacter calcoaceticus | |
2 | - |
D-galactose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
4 | - |
D-galactose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
5 | - |
D-galactose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
10 | - |
maltose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
11 | - |
maltose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
13 | - |
maltose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
25 | - |
D-glucose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
25 | - |
lactose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
26 | - |
lactose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
27 | - |
D-glucose | recombinant wild-type enzyme and tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
36 | - |
lactose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
41 | - |
3-O-methyl-D-glucose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
53 | - |
3-O-methyl-D-glucose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
56 | - |
3-O-methyl-D-glucose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
63 | - |
allose | recombinant wild-type enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
75 | - |
allose | recombinant tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus | |
76 | - |
allose | recombinant triple mutant enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | P13650 | - |
- |
Purification (Comment) | Organism |
---|---|
optimization of the purification process involving cation exchange chromatography in presence of Zn2+ | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | 3-O-methyl-D-glucono-1,5-lactone + ? | - |
? | |
allose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
D-galactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-galactono-1,5-lactone + ? | - |
? | |
D-glucose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ? | - |
? | |
D-glucose + ubiquinone | - |
Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
lactose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? | |
maltose + 2,6-dichlorophenolindolphenol | - |
Acinetobacter calcoaceticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PQQ glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
PQQGDH | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Acinetobacter calcoaceticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant tagged wild-type enzyme and mutant enzyme show about 90% remaining activity after 10 min, the nontagged wild-type enzyme shows about 40% remaining activity after 10 min | Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | i.e. PQQ | Acinetobacter calcoaceticus |