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Literature summary for 1.1.5.14 extracted from

  • Hui, H.; Huang, D.; McArthur, D.; Nissen, N.; Boros, L.G.; Heaney, A.P.
    Direct spectrophotometric determination of serum fructose in pancreatic cancer patients (2009), Pancreas, 38, 706-712.
    View publication on PubMed
Search for more literature for 1.1.5.14

Application

Application Comment Organism
biotechnology using fructose dehydrogenase-catalyzed conversion of d-fructose to 5-ketofructose, followed by quantitation of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] formazan production by direct spectrophotometry, an assay to measure serum fructose concentration is developed. The fructose dehydrogenase-based enzymatic assay correlates highly with gas chromatography-mass spectroscopic analysis of serum fructose. The assay is highly specific, exhibits no cross-reactivity with other sugars and is easy to perform Gluconobacter sp.

Organism

Organism UniProt Comment Textmining
Gluconobacter sp.
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] in the presence of phenazine methosulfate Gluconobacter sp. 5-dehydro-D-fructose + [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] formazan
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Synonyms

Synonyms Comment Organism
D-fructose dehydrogenase
-
 Gluconobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gluconobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Gluconobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 enzymatic activity is significantly inhibited at pH lower than 4.5 or higher than 6.5 Gluconobacter sp.