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Literature summary for 1.1.5.14 extracted from
- D-Fructose dehydrogenase from Gluconobacter industrius, membrane-bound (1982), Methods Enzymol., 89, 154-159.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| D-fructose, 5-10%, stabilizes | Gluconobacter oxydans |
| glycerol, 5-10%, stabilizes | Gluconobacter oxydans |
| sucrose, 5-10%, stabilizes | Gluconobacter oxydans |
| Triton X-100 is essential for preservation of purified enzyme | Gluconobacter oxydans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
D-fructose | - |
Gluconobacter oxydans |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | outer surface | Gluconobacter oxydans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19700 | - |
1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE | Gluconobacter oxydans |
| 50800 | - |
1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE | Gluconobacter oxydans |
| 67000 | - |
1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE | Gluconobacter oxydans |
| 140000 | - |
gel filtration | Gluconobacter oxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
IFO 3260 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 172 | - |
- |
Gluconobacter oxydans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or below, activity completely preserved | Gluconobacter oxydans |
| 0-4°C, pH 4.0-5.0, 0.1% Triton X-100, 1 mM 2-mercaptoethanol, for at least 2 weeks | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + 2,6-dichlorophenolindophenol | - |
Gluconobacter oxydans | 5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-fructose + ferricyanide | - |
Gluconobacter oxydans | 5-dehydro-D-fructose + ferrocyanide | - |
? | |
| D-fructose + nitro blue tetrazolium | - |
Gluconobacter oxydans | 5-dehydro-D-fructose + ? | - |
? | |
| D-fructose + phenazine methosulfate | - |
Gluconobacter oxydans | 5-dehydro-D-fructose + ? | - |
? | |
| additional information | no activity with NADP+ | Gluconobacter oxydans | ? | - |
? | |
| additional information | no activity with O2 | Gluconobacter oxydans | ? | - |
? | |
| additional information | no activity with NAD+ | Gluconobacter oxydans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 67000, flavin dehydrogenase, + 1 * 50800, cytochrome c, + 1 * 19700, subunit of unknown function, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | enzyme contains a cytochrome c of 50800 Da | Gluconobacter oxydans | |
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Release 2023.1 (January 2023)
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