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Literature summary for 1.1.5.13 extracted from

  • Huedig, M.; Maier, A.; Scherrers, I.; Seidel, L.; Jansen, E.E.; Mettler-Altmann, T.; Engqvist, M.K.; Maurino, V.G.
    Plants possess a cyclic mitochondrial metabolic pathway similar to the mammalian metabolic repair mechanism involving malate dehydrogenase and L-2-hydroxyglutarate dehydrogenase (2015), Plant Cell Physiol., 56, 1820-1830 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
L-2-hydroxyglutarate at pH 7.4 and 30┬░C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-2-hydroxyglutarate + a quinone Arabidopsis thaliana
-
2-oxoglutarate + a quinol
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Columbia-0
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
520
-
at pH 7.4 and 30┬░C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-2-hydroxyglutarate + 2,6-dichlorophenolindophenol 3.7% activity compared to L-2-hydroxyglutarate Arabidopsis thaliana 2-oxoglutarate + reduced 2,6-dichlorophenolindophenol
-
?
L-2-hydroxyglutarate + 2,6-dichlorophenolindophenol
-
Arabidopsis thaliana 2-oxoglutarate + reduced 2,6-dichlorophenolindophenol
-
?
L-2-hydroxyglutarate + a quinone
-
Arabidopsis thaliana 2-oxoglutarate + a quinol
-
?
additional information the enzyme is not active in the presence of NAD+, NADP+, cytochrome c or O2. No activity with glycolate, L-lactate or D-lactate Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
L-2-hydroxyglutarate dehydrogenase
-
Arabidopsis thaliana
L-2HG dehydrogenase
-
Arabidopsis thaliana
L-2HGDH
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
613
-
L-2-hydroxyglutarate at pH 7.4 and 30┬░C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 more than 50% activity between pH 6.5 and 8.5 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains FAD. The addition of 2.5 mM FAD results in 31% increase of activity as measured without FAD Arabidopsis thaliana