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Literature summary for 1.1.5.13 extracted from
- Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase (2008), J. Bacteriol., 190, 3793-3798 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C41(DE3) cells | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.095 | - |
L-2-hydroxyglutarate | at pH 7.0 and 25°C | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2-hydroxyglutarate + O2 | Escherichia coli | - |
2-oxoglutarate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA-Sepharose 6 column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2-hydroxyglutarate + O2 | - |
Escherichia coli | 2-oxoglutarate + H2O2 | - |
? | |
| additional information | no activity with dimethylglycine, sarcosine, butyraldehyde or 3-hydroxybutyric acid. Most 2-hydroxy acids are ineffective as substrates, including L-malic acid, DL-malic acid, DL-lactic acid, L-mandelic acid, D-mandelic acid, and 2-hydroxycaproic acid | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47640, His-tagged protein, calculated from amino acid sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-2-hydroxyglutarate oxygenase | - |
Escherichia coli |
| lhgO | - |
Escherichia coli |
| YgaF | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
L-2-hydroxyglutarate | at pH 7.0 and 25°C | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | the enzyme contains a noncovalently bound FAD | Escherichia coli | |
| additional information | not affected by NAD+ or NADP+ | Escherichia coli |
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