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Literature summary for 1.1.5.12 extracted from
- Mechanisms of active transport in isolated bacterial membrane vesicles (1973), J. Biol. Chem., 248, 7012-7017.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-hydroxy-3-butynoic acid | inactivation | Escherichia coli |  |
| additional information | not inhibited by 4-chloromercuribenzoate, N-ethylmaleimide, or 5,5'-dithiobis(2-nitrobenzoic acid) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
D-lactate | presence of Triton X-100, pH 7.8, 25°C | Escherichia coli | |
| 1.4 | - |
D-lactate | pH 7.8, 25°C | Escherichia coli | |
| 21 | - |
L-lactate | presence of Triton X-100, pH 7.8, 25°C | Escherichia coli | |
| 30 | - |
L-lactate | pH 7.8, 25°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 75000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli ML 308-225 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-lactate + 2,6-dichloroindophenol | - |
Escherichia coli | pyruvate + reduced 2,6-dichloroindophenol | - |
? | |
| D-lactate + 2,6-dichloroindophenol | - |
Escherichia coli ML 308-225 | pyruvate + reduced 2,6-dichloroindophenol | - |
? | |
| D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium | - |
Escherichia coli | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium | - |
? | |
| D-lactate + oxidized 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium | - |
Escherichia coli ML 308-225 | pyruvate + reduced 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium | - |
? | |
| L-lactate + 2,6-dichloroindophenol | - |
Escherichia coli | pyruvate + reduced 2,6-dichloroindophenol | - |
? | |
| L-lactate + 2,6-dichloroindophenol | - |
Escherichia coli ML 308-225 | pyruvate + reduced 2,6-dichloroindophenol | - |
? | |
| additional information | not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate | Escherichia coli | ? | - |
? | |
| additional information | not active with D-glycerate, L-glycerate, succinate, malate, D-tartrate, L-tartrate, meso-tartrate, 1-propanol, or isopropanol as substrates and oxidized diphosphopyridine nucleotide has no effect on the catalytic conversion of D-lactate to pyruvate | Escherichia coli ML 308-225 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Escherichia coli |
| 8 | 9.5 | presence of Triton X-100 | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1 mol of FAD per mol of enzyme | Escherichia coli | |
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Release 2023.1 (January 2023)
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