Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cyanide | stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | stimulates D-lactate dehydrogenase activity at neutral pH, though it inhibits the activity at alkaline pH. At 1.5 mM, 50% inhibition at pH 9.3 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
(R)-lactate | pH 8.0, 23°C | Escherichia coli | |
1.2 | - |
(R,S)-2-hydroxybutanoate | pH 8.0, 23°C | Escherichia coli | |
1.4 | - |
(R)-lactate | presence of cyanide, pH 8.0, 23°C | Escherichia coli | |
18 | - |
(S)-lactate | pH 8.0, 23°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
sucrose density gradient centrifugation | Escherichia coli |
72000 | - |
1 * 72000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-90°C, 20% ethylene glycol, stable for at least 2 weeks | Escherichia coli |
0°C, 5 days, 80% residual activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-lactate + phenazine methosulfate | - |
Escherichia coli | pyruvate + reduced phenazine methosulfate | - |
? | |
(R,S)-2-hydroxybutanoate + a quinone | 12% of the activity with (R)-lactate | Escherichia coli | 2-oxobutanoate + a quinol | - |
? | |
(S)-lactate + oxidized dichlorophenolindophenol | 14% of the activity with (R)-lactate | Escherichia coli | pyruvate + reduced dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli |