Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloramine T | 60% inhibition at 1 mM | Penicillium sp. | |
Hg2+ | 60% inhibition at 1 mM | Penicillium sp. | |
hydroxylamine | 87% inhibition at 1 mM | Penicillium sp. | |
KCN | complete inhibition at 1 mM | Penicillium sp. | |
additional information | EDTA, phenylglycoxyl, iodoacetic acid, and o-phenanthroline do not show significant inhibitory effects on the enzyme activity | Penicillium sp. | |
NaN3 | complete inhibition at 1 mM | Penicillium sp. | |
Tetranitromethane | 46% inhibition at 1 mM | Penicillium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Penicillium sp. | 9986 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 15% increase of activity in the presence of 1 mM Cu2+ | Penicillium sp. | |
Fe3+ | 10% increase of activity in the presence of 1 mM Fe3+ | Penicillium sp. | |
additional information | not influenced by Co2+, Mn2+, Mg2+, Pb2+, Ca2+, and Cd2+ | Penicillium sp. | |
Zn2+ | 15% increase of activity in the presence of 1 mM Zn2+ | Penicillium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
400000 | - |
gel filtration | Penicillium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium sp. | - |
- |
- |
Penicillium sp. TS-622 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, Sephadex G-100 gel filtration, Fractogel DEAE-650M column chromatography, Toyopearl phenyl-650M column chromatography, and Ultrogel hydroxyapatite column chromatography | Penicillium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Penicillium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
crude extract, at pH 7.0 and 30°C | Penicillium sp. |
66.7 | - |
after 39.2fold purification, at pH 7.0 and 30°C | Penicillium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone + O2 | 132% activity compared to glycerol | Penicillium sp. | 3-hydroxy-2-oxopropanal + H2O2 | - |
? | |
dihydroxyacetone + O2 | 132% activity compared to glycerol | Penicillium sp. TS-622 | 3-hydroxy-2-oxopropanal + H2O2 | - |
? | |
glycerol + O2 | 100% activity | Penicillium sp. | glyceraldehyde + H2O2 | - |
? | |
glycerol + O2 | 100% activity | Penicillium sp. TS-622 | glyceraldehyde + H2O2 | - |
? | |
additional information | the enzyme does not oxidize 1,2-propanediol, 1,3-butanediol, glyceraldehyde, glycero-3-phosphate, 3-phosphoglyceric acid, methanol, ethanol, n-propanol, iso-propanol, n-butanol, ethylene glycol, D-glucose, D-galactose, and phenol | Penicillium sp. | ? | - |
? | |
additional information | the enzyme does not oxidize 1,2-propanediol, 1,3-butanediol, glyceraldehyde, glycero-3-phosphate, 3-phosphoglyceric acid, methanol, ethanol, n-propanol, iso-propanol, n-butanol, ethylene glycol, D-glucose, D-galactose, and phenol | Penicillium sp. TS-622 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Penicillium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | after 1 h of incubation, the enzyme is relatively stable from 20 to 40°C and loses most of its activity over 50°C | Penicillium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Penicillium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | the enzyme is stable in the pH range from 5.5 to 6.5 | Penicillium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Penicillium sp. |