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Literature summary for 1.1.3.9 extracted from

  • Paukner, R.; Staudigl, P.; Choosri, W.; Haltrich, D.; Leitner, C.
    Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli (2015), Protein Expr. Purif., 108, 73-79 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.9

Activating Compound

Activating Compound Comment Organism Structure
Tween 80 slight activation Fusarium sambucinum

Cloned(Commentary)

Cloned (Comment) Organism
gene gaoA, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Fusarium sambucinum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 56% inhibition Fusarium sambucinum
KCN complete inhibition Fusarium sambucinum
NaN3 complete inhibition Fusarium sambucinum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no or poor effects by Mg2+, K+, Na+, NH4+, Mn2+, and NaF Fusarium sambucinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galactose + O2 Fusarium sambucinum
-
 D-galacto-hexodialdose + H2O2
-
 ?
D-galactose + O2 Fusarium sambucinum MA1886
-
 D-galacto-hexodialdose + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium sambucinum A0A089QAB6 i.e. Gibberella pulicaris
-
Fusarium sambucinum MA1886 A0A089QAB6 i.e. Gibberella pulicaris
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme 204fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Fusarium sambucinum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
159
-
 purified recombinant His-tagged enzyme, substrate D-galactose, pH 7.0, 30°C Fusarium sambucinum
226
-
 purified recombinant His-tagged enzyme, substrate 1-methyl-beta-D-galactopyranoside, pH 7.0, 30°C Fusarium sambucinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-beta-D-galactopyranoside + O2 high activity Fusarium sambucinum ? + H2O2
-
 ?
1-methyl-beta-D-galactopyranoside + O2 high activity Fusarium sambucinum MA1886 ? + H2O2
-
 ?
D-galactose + O2
-
 Fusarium sambucinum D-galacto-hexodialdose + H2O2
-
 ?
D-galactose + O2
-
 Fusarium sambucinum MA1886 D-galacto-hexodialdose + H2O2
-
 ?
lactose + O2
-
 Fusarium sambucinum ? + H2O2
-
 ?
lactose + O2
-
 Fusarium sambucinum MA1886 ? + H2O2
-
 ?
melibiose + O2 high catalytic efficiency Fusarium sambucinum ? + H2O2
-
 ?
melibiose + O2 high catalytic efficiency Fusarium sambucinum MA1886 ? + H2O2
-
 ?
additional information standard ABTS assay. The enzyme is highly specific for molecular oxygen as an electron acceptor, and shows no appreciable activity with a range of alternative acceptors investigated, no activity with ABTS cation radical, ferrocenium ion, 1,4-benzoquinone, 2,6-dichloro-indophenol (DCIP), ferricyanide, guaiacol radical, 2,6-dimethoxyphenol radical, caffeic acid radical, p-coumaric acid radical, ferulic acid radical, sinapic acid radical, thioflavin T, 2-(4'-methylaminophenyl)benzothiazole and 1,10-diethyl-2,20-carbocyanine iodide Fusarium sambucinum ?
-
 ?
additional information standard ABTS assay. The enzyme is highly specific for molecular oxygen as an electron acceptor, and shows no appreciable activity with a range of alternative acceptors investigated, no activity with ABTS cation radical, ferrocenium ion, 1,4-benzoquinone, 2,6-dichloro-indophenol (DCIP), ferricyanide, guaiacol radical, 2,6-dimethoxyphenol radical, caffeic acid radical, p-coumaric acid radical, ferulic acid radical, sinapic acid radical, thioflavin T, 2-(4'-methylaminophenyl)benzothiazole and 1,10-diethyl-2,20-carbocyanine iodide Fusarium sambucinum MA1886 ?
-
 ?
raffinose + O2
-
 Fusarium sambucinum 6''-aldehydoraffinose + 6''-carboxyraffinose + H2O2 + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 68500, recombinant His6-tagged enzyme, SDS-PAGE Fusarium sambucinum
More three-dimensional structure modeling based on the structure of GalOx from Fusarium graminearum Fusarium sambucinum

Synonyms

Synonyms Comment Organism
GalOx
-
 Fusarium sambucinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 recombinant His-tagged enzyme Fusarium sambucinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the purified recombinant His-tagged enzyme shows half-lifes of 11.2 min, 5.3 min, and 2.7 min for incubation at 40°C, 50°C, and 60°C, respectively Fusarium sambucinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5 recombinant His-tagged enzyme Fusarium sambucinum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 activity range, more than 95% activity in the range of pH 6.0-7.5, profile overview. Inactive at pH 5.0 and below Fusarium sambucinum

General Information

General Information Comment Organism
additional information three-dimensional structure modeling based on the structure of GalOx from Fusarium graminearum Fusarium sambucinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2700
-
 melibiose recombinant His-tagged enzyme, pH 7.0, 30°C Fusarium sambucinum