Application | Comment | Organism |
---|---|---|
medicine | a gene therapy approach can be successfully employed in the treatment and further study of vitamin C deficiency in scurvy-prone mammals | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P58710 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
GULO | - |
Mus musculus |
gulonolactone oxidase | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | a deficiency in GULO expression results in the inability to produce vitamin C. Using a previously derived Gulo-expressing vector, which produces murine GULO under the control of the murine cytomegalovirus (mCMV) promoter, a recombinant helper-dependent adenovirus (HDAd-mCMV-Gulo) is constructed that can be used to correct this genetic defect. A human liver cell line (Hep G2) infected with the HDAd-mCMV-Gulo vector expresses GULO in a time- and gene dose-dependent manner. These cells also produce ascorbic acid when exogenous gulonolactone is supplemented in the medium. Likewise, Gulo(-/-) mice treated with HDAd-mCMV-Gulo express GULO in the liver and produce ascorbic acid | Mus musculus |