Application | Comment | Organism |
---|---|---|
synthesis | AAO is able to produce 2,5-furandicarboxylic acid from formylfurancarboxylic acid, allowing full oxidation of 5-hydroxymethylfurfural. During 5-hydroxymethylfurfural reactions, an inhibitory effect of the H2O2 produced in the first two oxidation steps is the cause of the lack of AAO activity on formylfurancarboxylic acid. 5-Hydroxymethylfurfural is successfully converted into 2,5-furandicarboxylic acid when the AAO reaction is carried out in the presence of catalase | Pleurotus eryngii |
Protein Variants | Comment | Organism |
---|---|---|
F397Y | mutant shows improved production of 2,5-furandicarboxylic acid, with 70% yield | Pleurotus eryngii |
F501H | mutant shows improved production of 2,5-furandicarboxylic acid, with 97% yield | Pleurotus eryngii |
I500M | mutant shows improved production of 2,5-furandicarboxylic acid, with 80% yield | Pleurotus eryngii |
I500M/F501 W | mutant shows improved production of 2,5-furandicarboxylic acid, reaching a total turnover number over 16,000 in presence of 15 mM 5-hydroxymethylfurfural | Pleurotus eryngii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibitory to the oxidation of formylfurancarboxylic acid, oxidation activities of AAO on 5-hydroxymethylfurfural and 2,5-diformylfuran are independent of the presence of H2O2 | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 6 mM formylfurancarboxylic acid | Pleurotus eryngii | |
0.031 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 15 mM H2O2 | Pleurotus eryngii | |
0.0318 | - |
4-methoxybenzyl alcohol | pH 6, 25°C | Pleurotus eryngii | |
0.0335 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 1 mM H2O2 | Pleurotus eryngii | |
0.038 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 0.8 mM formylfurancarboxylic acid | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94219 | cf. EC 1.1.3.47 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-diformylfuran + O2 | - |
Pleurotus eryngii | formylfurancarboxylic acid + ? | - |
? | |
4-anisaldehyde + O2 | - |
Pleurotus eryngii | 4-anisic acid + H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-methoxybenzyl aldehyde + H2O2 | - |
? | |
5-hydroxymethylfurfural + O2 | - |
Pleurotus eryngii | 2,5-diformylfuran + H2O2 | - |
? | |
formylfurancarboxylic acid + O2 | - |
Pleurotus eryngii | 2,5-furandicarboxylic acid + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-anisaldehyde | pH 6, 25°C, presence of 1 mM H2O2 | Pleurotus eryngii | |
0.11 | - |
4-anisaldehyde | pH 6, 25°C, presence of 15 mM H2O2 | Pleurotus eryngii | |
0.12 | - |
4-anisaldehyde | pH 6, 25°C | Pleurotus eryngii | |
1.03 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 6 mM formylfurancarboxylic acid | Pleurotus eryngii | |
1.32 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 0.8 mM formylfurancarboxylic acid | Pleurotus eryngii | |
97 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 15 mM H2O2 | Pleurotus eryngii | |
104 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 1 mM H2O2 | Pleurotus eryngii | |
108 | - |
4-methoxybenzyl alcohol | pH 6, 25°C | Pleurotus eryngii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2080 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 0.8 mM formylfurancarboxylic acid | Pleurotus eryngii | |
2250 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 6 mM formylfurancarboxylic acid | Pleurotus eryngii | |
3100 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 1 mM H2O2 | Pleurotus eryngii | |
3120 | - |
4-methoxybenzyl alcohol | pH 6, 25°C, presence of 15 mM H2O2 | Pleurotus eryngii | |
3390 | - |
4-methoxybenzyl alcohol | pH 6, 25°C | Pleurotus eryngii |