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Literature summary for 1.1.3.7 extracted from

  • Gomez De Santos, P.; Lazaro, S.; Vin-Gonzalez, J.; Hoang, M.; Sanchez-Moreno, I.; Glieder, A.; Hollmann, F.; Alcalde, M.
    Evolved peroxygenase-aryl alcohol oxidase fusions for self-sufficient oxyfunctionalization reactions (2020), ACS Catal., 10, 13524-13534 .
No PubMed abstract available
Search for more literature for 1.1.3.7

Application

Application Comment Organism
synthesis construction of fusion proteins with Saccharomyces cerevisiae unspecific peroxidase, EC 1.11.2.1, using different peptide linkers. The reaction system is optimized to control the aromatic alcohol transformation rate, and therefore the H2O2 supply, to achieve total turnover numbers of 62000 for the biocatalytic synthesis of dextrorphan, a metabolite of the antitussive drug dextromethorphan Komagataella pastoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0204
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)17, pH 4, 25°C Komagataella pastoris
0.0214
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (AP)5(GGGGS)1, pH 4, 25°C Komagataella pastoris
0.0215
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (AP)15(GGGGS)2, pH 4, 25°C Komagataella pastoris
0.023
-
 (R,S)-4-methoxybenzyl alcohol free enzyme, pH 4, 25°C Komagataella pastoris
0.028
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)9, pH 4, 25°C Komagataella pastoris
0.0382
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)12, pH 4, 25°C Komagataella pastoris

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-4-methoxybenzyl alcohol + O2
-
 Komagataella pastoris 1-(4-methoxyphenyl)ethanol + H2O2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)12, pH 4, 25°C Komagataella pastoris
30
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (AP)5(GGGGS)1, pH 4, 25°C Komagataella pastoris
30
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)9, pH 4, 25°C Komagataella pastoris
34
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (AP)15(GGGGS)2, pH 4, 25°C Komagataella pastoris
41
-
 (R,S)-4-methoxybenzyl alcohol free enzyme, pH 4, 25°C Komagataella pastoris
44
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)17, pH 4, 25°C Komagataella pastoris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
747
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)12, pH 4, 25°C Komagataella pastoris
1061
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)9, pH 4, 25°C Komagataella pastoris
1383
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (AP)5(GGGGS)1, pH 4, 25°C Komagataella pastoris
1568
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (AP)15(GGGGS)2, pH 4, 25°C Komagataella pastoris
1782
-
 (R,S)-4-methoxybenzyl alcohol free enzyme, pH 4, 25°C Komagataella pastoris
2183
-
 (R,S)-4-methoxybenzyl alcohol protein fused to peroxidase, linker (GGGGS)17, pH 4, 25°C Komagataella pastoris