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Literature summary for 1.1.3.7 extracted from
- Characterization of a new aryl-alcohol oxidase secreted by the phytopathogenic fungus Ustilago maydis (2016), Appl. Microbiol. Biotechnol., 100, 697-706 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis, recombinant expression in Pichia pastoris | Ustilago maydis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Hydroxybenzyl alcohol | shows substrate inhibition at higher concentration | Ustilago maydis |  |
| anisyl alcohol | shows substrate inhibition at higher concentration | Ustilago maydis | |
| cinnamyl alcohol | shows substrate inhibition at higher concentration | Ustilago maydis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Ustilago maydis | |
| 0.0048 | - |
anisyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 0.015 | - |
2,4-hexadien-1-ol | pH 6.0, 30°C | Ustilago maydis | |
| 0.035 | - |
cinnamyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 0.042 | - |
4-Hydroxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 0.12 | - |
veratryl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 1.82 | - |
2,4-Dimethoxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Ustilago maydis | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ustilago maydis | - |
strains BRFM 1093 and 521/FGSC 9021 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the enzyme contains no putative N/O-glycosylation sites | Ustilago maydis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | Ustilago maydis grown on maize | Ustilago maydis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dimethoxybenzyl alcohol + O2 | - |
Ustilago maydis | 2,4-dimethoxybenzaldehyde + H2O2 | - |
? | |
| 2,4-hexadien-1-ol + O2 | - |
Ustilago maydis | ? + H2O2 | - |
? | |
| 4-hydroxybenzyl alcohol + O2 | - |
Ustilago maydis | 4-hydroxybenzaldehyde + H2O2 | - |
? | |
| 4-methoxybenzyl alcohol + O2 | - |
Ustilago maydis | 4-methoxybenzaldehyde + H2O2 | - |
? | |
| anisyl alcohol + O2 | best substrate | Ustilago maydis | anisaldehyde + H2O2 | - |
? | |
| benzyl alcohol + O2 | - |
Ustilago maydis | benzaldehyde + H2O2 | - |
? | |
| cinnamyl alcohol + O2 | - |
Ustilago maydis | cinnamaldehyde + H2O2 | - |
? | |
| additional information | the enzyme is able to oxidize several aromatic alcohols. Of the tested aryl-alcohols, the highest oxidation rate is obtained with 4-anisyl alcohol. Oxygen, 1,4-benzoquinone, and 2,6-dichloroindophenol can serve as electron acceptors. Assay method using ABTS [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)]/horseradish peroxidase. The enzyme shows no activity as a GMC oxidoreductase | Ustilago maydis | ? | - |
? | |
| veratryl alcohol + O2 | i.e. 3,4-dimethoxybenzyl alcohol | Ustilago maydis | veratrylaldehyde + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme homology structure modeling | Ustilago maydis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAO | - |
Ustilago maydis |
| um04044 | - |
Ustilago maydis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ustilago maydis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
2,4-Dimethoxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 45 | - |
anisyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 53 | - |
veratryl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 64 | - |
2,4-hexadien-1-ol | pH 6.0, 30°C | Ustilago maydis | |
| 75 | - |
4-Hydroxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 88 | - |
cinnamyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Ustilago maydis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Ustilago maydis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the AA3_2 subfamily, phylogenetic analysis | Ustilago maydis |
| additional information | enzyme homology structure modeling | Ustilago maydis |
| physiological function | the aryl-alcohol oxidase acts on the lignin fraction in biomass | Ustilago maydis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.5 | - |
2,4-Dimethoxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 440 | - |
veratryl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 1790 | - |
4-Hydroxybenzyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 2510 | - |
cinnamyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
| 4270 | - |
2,4-hexadien-1-ol | pH 6.0, 30°C | Ustilago maydis | |
| 9380 | - |
anisyl alcohol | pH 6.0, 30°C | Ustilago maydis | |
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