Protein Variants | Comment | Organism |
---|---|---|
F501A | the AAO preference for (S)-1-(4-fluorophenyl)ethanol is increased threefold when the bulky side chain of Phe501 is removed in the F501A variant, which shows a stereoselectivity S/R ratio of 66:1 for this secondary alcohol | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady state and transient state kinetic constants for alcohol and O2 of AAO oxidation of a deuterated and normal (alpha-protiated) 4-methoxybenzyl alcohol, solvent kinetic isotope effects, overview | Pleurotus eryngii | |
0.025 | - |
4-methoxybenzyl alcohol | substrate alpha-deuterated 4-methoxybenzyl alcohol, pH 6.0, 25°C | Pleurotus eryngii | |
0.049 | - |
4-methoxybenzyl alcohol | substrate 4-methoxybenzyl alcohol, pH 6.0, 25°C | Pleurotus eryngii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-4-methoxybenzyl alcohol + O2 | Pleurotus eryngii | over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective | 1-(4-methoxyphenyl)ethanol + H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | Pleurotus eryngii | - |
4-methoxybenzaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94219 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 | oxidation mechanism by AAO, overview | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-4-methoxybenzyl alcohol + O2 | over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective | Pleurotus eryngii | 1-(4-methoxyphenyl)ethanol + H2O2 | - |
? | |
(S)-1-(4-fluorophenyl)ethanol + O2 | mutant F501A | Pleurotus eryngii | 1-(4-fluorophenyl)acetaldehyde + H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | - |
Pleurotus eryngii | 4-methoxybenzaldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAO | - |
Pleurotus eryngii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pleurotus eryngii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
4-methoxybenzyl alcohol | substrate alpha-deuterated 4-methoxybenzyl alcohol, pH 6.0, 25°C | Pleurotus eryngii | |
196 | - |
4-methoxybenzyl alcohol | substrate 4-methoxybenzyl alcohol, pH 6.0, 25°C | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Pleurotus eryngii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pleurotus eryngii |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glucose methanol choline oxidase superfamily | Pleurotus eryngii |
additional information | docking of 4-methoxybenzyl alcohol at the buried crystal active site, and quantum mechanical/molecular mechanical study, overview | Pleurotus eryngii |
physiological function | the enzyme provides H2O2 to ligninolytic peroxidases | Pleurotus eryngii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1020 | - |
4-methoxybenzyl alcohol | substrate alpha-deuterated 4-methoxybenzyl alcohol, pH 6.0, 25°C | Pleurotus eryngii | |
3980 | - |
4-methoxybenzyl alcohol | substrate 4-methoxybenzyl alcohol, pH 6.0, 25°C | Pleurotus eryngii |