Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chrysomela tremula |
expression in Escherichia coli | Chrysomela populi |
expression of the constructs encoding CpSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO | Chrysomela populi |
expression of the constructs encoding CtSAO in Escherichia coli BL21(DE3) results in the formation of insoluble inclusion bodies. The expression construct is coexpressed with the plasmid pREP4-groESL, encoding chaperonine proteins of the Hsp60 family (GroEL and GroES). Coexpression at 15°C for 16 h results in the formation of soluble recombinant SAO | Chrysomela tremula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxybenzyl alcohol | substrate inhibition at concentrations of about 250 mM | Chrysomela populi | |
2-hydroxybenzyl alcohol | substrate inhibition at concentrations of about 250 mM | Chrysomela tremula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28 | - |
2-hydroxybenzyl alcohol | pH 6.0 | Chrysomela populi | |
40 | - |
2-hydroxybenzyl alcohol | pH 6.0 | Chrysomela tremula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Chrysomela tremula | - |
- |
extracellular | - |
Chrysomela populi | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
apparent mass of about 80 kDa determined by SDS-PAGE, molecular mass of 67.7 kDa deduced from sequence | Chrysomela populi |
66700 | - |
x * 66700, CpSAP without signal peptide, calculated from sequence | Chrysomela populi |
67100 | - |
x * 67100, CtSAO without signal peptide, calculated from sequence | Chrysomela tremula |
67700 | - |
deduced from sequence | Chrysomela tremula |
67700 | - |
deduced from sequence | Chrysomela populi |
80000 | - |
apparent mass of about 80 kDa determined by SDS-PAGE | Chrysomela tremula |
80000 | - |
apparent mass of about 80 kDa determined by SDS-PAGE | Chrysomela populi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybenzyl alcohol + O2 | Chrysomela tremula | essential for the activation of the plant derived precursor salicin | 2-hydroxybenzaldehyde + H2O2 | - |
? | |
2-hydroxybenzyl alcohol + O2 | Chrysomela populi | essential for the activation of the plant derived precursor salicin | 2-hydroxybenzaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chrysomela populi | B4F335 | - |
- |
Chrysomela tremula | B4F334 | - |
- |
Chrysomela tremula | B4F334 | encoded by sao gene | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Chrysomela tremula |
glycoprotein | presence of hybrid type structures (GlcNAc Man5-6 GlcNAc2) | Chrysomela populi |
glycoprotein | N-glycosylation predicted by data obtained from microsequencing | Chrysomela tremula |
glycoprotein | N-glycosylation predicted by data obtained from microsequencing | Chrysomela populi |
Purification (Comment) | Organism |
---|---|
- |
Chrysomela tremula |
- |
Chrysomela populi |
gel filtration | Chrysomela tremula |
gel filtration | Chrysomela populi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 | belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) | Chrysomela tremula | |
an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 | belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) | Chrysomela populi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | - |
Chrysomela tremula | - |
adult | - |
Chrysomela populi | - |
exocrine glandular secretion | from larva | Chrysomela tremula | - |
exocrine glandular secretion | from larva | Chrysomela populi | - |
larva | - |
Chrysomela tremula | - |
larva | - |
Chrysomela populi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cloning and microsequencing of peptides, substrate specificity of recombinant and native enzyme, phylogeny and comparison of sequence similarities | Chrysomela populi |
additional information | - |
cloning and microsequencing of peptides, substrate specificity of recombinant in comparison with native enzyme, phylogeny and comparison of sequence similarities | Chrysomela tremula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybenzyl alcohol + O2 | - |
Chrysomela tremula | 2-hydroxybenzaldehyde + H2O2 | - |
? | |
2-hydroxybenzyl alcohol + O2 | - |
Chrysomela populi | 2-hydroxybenzaldehyde + H2O2 | - |
? | |
2-hydroxybenzyl alcohol + O2 | i.e. salicyl alcohol | Chrysomela tremula | 2-hydroxybenzaldehyde + H2O2 | - |
? | |
2-hydroxybenzyl alcohol + O2 | i.e. salicyl alcohol | Chrysomela populi | 2-hydroxybenzaldehyde + H2O2 | - |
? | |
2-hydroxybenzyl alcohol + O2 | essential for the activation of the plant derived precursor salicin | Chrysomela tremula | 2-hydroxybenzaldehyde + H2O2 | - |
? | |
2-hydroxybenzyl alcohol + O2 | essential for the activation of the plant derived precursor salicin | Chrysomela populi | 2-hydroxybenzaldehyde + H2O2 | - |
? | |
2-methoxybenzyl alcohol + O2 | recombinant enzyme shows 2.3% of the activity with 2-hydroxybenzyl alcohol | Chrysomela tremula | 2-methoxybenzylaldehyde + H2O | - |
? | |
2-methoxybenzyl alcohol + O2 | recombinant enzyme shows 3.2% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 14% of the activity with 2-hydroxybenzyl alcohol | Chrysomela populi | 2-methoxybenzylaldehyde + H2O | - |
? | |
2-methylbenzyl alcohol + O2 | recombinant enzyme shows 19.1% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 21% of the activity with 2-hydroxybenzyl alcohol | Chrysomela populi | 2-methylbenzylaldehyde + H2O | - |
? | |
2-methylbenzyl alcohol + O2 | recombinant enzyme shows 21.9% of the activity with 2-hydroxybenzyl alcohol | Chrysomela tremula | 2-methylbenzylaldehyde + H2O | - |
? | |
2-phenylethyl alcohol + O2 | recombinant enzyme shows 1.2% of the activity with 2-hydroxybenzyl alcohol | Chrysomela tremula | 2-phenylacetaldehyde + H2O | - |
? | |
2-phenylethyl alcohol + O2 | recombinant enzyme shows 3.8% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 21% of the activity with 2-hydroxybenzyl alcohol | Chrysomela populi | 2-phenylacetaldehyde + H2O | - |
? | |
3-hydroxybenzyl alcohol + O2 | recombinant enzyme shows 13.1% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows less than 10% of the activity with 2-hydroxybenzyl alcohol | Chrysomela populi | 3-hydroxybenzaldehyde + H2O2 | - |
? | |
3-hydroxybenzyl alcohol + O2 | recombinant enzyme shows 16.2% of the activity with 2-hydroxybenzyl alcohol | Chrysomela tremula | 3-hydroxybenzaldehyde + H2O2 | - |
? | |
3-methoxybenzyl alcohol + O2 | recombinant enzyme shows 1% of the activity with 2-hydroxybenzyl alcohol | Chrysomela tremula | 3-methoxybenzylaldehyde + H2O2 | - |
? | |
4-hydroxybenzyl alcohol + O2 | recombinant enzyme shows 2.7% of the activity with 2-hydroxybenzyl alcohol | Chrysomela tremula | 4-hydroxybenzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | recombinant enzyme shows 12.2% of the activity with 2-hydroxybenzyl alcohol, native enzyme shows 23% of the activity with 2-hydroxybenzyl alcohol | Chrysomela populi | benzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | recombinant enzyme shows 23.2% of the activity with 2-hydroxybenzyl alcohol | Chrysomela tremula | benzaldehyde + H2O2 | - |
? | |
additional information | belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) | Chrysomela tremula | ? | - |
? | |
additional information | belongs to the GMC oxidoreductase family (GMC: glucose-methanol-choline) | Chrysomela populi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Chrysomela tremula |
? | x * 80000, SDS-PAGE | Chrysomela populi |
? | x * 66700, CpSAP without signal peptide, calculated from sequence | Chrysomela populi |
? | x * 67100, CtSAO without signal peptide, calculated from sequence | Chrysomela tremula |
Synonyms | Comment | Organism |
---|---|---|
CpSAO | - |
Chrysomela populi |
CtSAO | - |
Chrysomela tremula |
salicyl alcohol oxidase | - |
Chrysomela tremula |
salicyl alcohol oxidase | - |
Chrysomela populi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Chrysomela populi | calculated from sequence | - |
6.1 |