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Literature summary for 1.1.3.6 extracted from
- Production and optimization of cholesterol oxidase from Rhodococcus species (2015), Int. J. Pharm. Pharm. Sci., 7, 265-268 .
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Rhodococcus sp. | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | Rhodococcus sp. | - |
cholest-4-en-3-one + H2O2 | - |
? |  |
| cholesterol + O2 | Rhodococcus sp. COP 2 | - |
cholest-4-en-3-one + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus sp. | - |
isolated from urban compost and dairy soil samples | - |
| Rhodococcus sp. COP 2 | - |
isolated from urban compost and dairy soil samples | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimization of conditions for cholesterol oxidase production by the microorganism isolated from urban compost and dairy soil samples, optimal conditions for production of enzyme CHO from Rhodococcus sp. strain COP2 are pH 7.5 and 35°C, effect of inorganic and organic nitrogen sources on enzyme production, overview | Rhodococcus sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1162 U/ml, crude enzyme, pH 7.5, 35°C | Rhodococcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | - |
Rhodococcus sp. | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | - |
Rhodococcus sp. COP 2 | cholest-4-en-3-one + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHO | - |
Rhodococcus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Rhodococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Rhodococcus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Rhodococcus sp. | |
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Release 2023.1 (January 2023)
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