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Literature summary for 1.1.3.6 extracted from
- Cholesterol oxidases: properties and applications (1976), J. Steroid Biochem., 7, 705-713.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
5alpha-Cholestan-3beta-ol | - |
Nocardia erythropolis |  |
| 0.0029 | - |
cholesterol | - |
Nocardia erythropolis | |
| 0.0059 | - |
stigmasterol | - |
Nocardia erythropolis | |
| 0.0066 | - |
sitosterol | - |
Nocardia erythropolis | |
| 0.0115 | - |
fucosterol | - |
Nocardia erythropolis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + O2 | Nocardia erythropolis | first step in cholesterol degradation | cholest-5-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | Streptomyces violascens | first step in cholesterol degradation | cholest-5-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | Brevibacterium sterolicum | first step in cholesterol degradation | cholest-5-en-3-one + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacterium sterolicum | - |
- |
- |
| Nocardia erythropolis | - |
- |
- |
| Streptomyces violascens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overview: purification procedures | Nocardia erythropolis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Nocardia erythropolis | |
| cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Streptomyces violascens | |
| cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Brevibacterium sterolicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-cholestan-3beta-ol + O2 | - |
Streptomyces violascens | 5alpha-cholestan-3-one + H2O2 | - |
? | |
| 5alpha-cholestan-3beta-ol + O2 | - |
Brevibacterium sterolicum | 5alpha-cholestan-3-one + H2O2 | - |
? | |
| 5alpha-cholestan-3beta-ol + O2 | 87% of activity | Nocardia erythropolis | 5alpha-cholestan-3-one + H2O2 | - |
? | |
| 7E,22-ergostadien-3beta-ol + O2 | - |
Nocardia erythropolis | 7E,22-ergostadien-3-one + H2O2 | - |
? | |
| 7E,22-ergostadien-3beta-ol + O2 | - |
Streptomyces violascens | 7E,22-ergostadien-3-one + H2O2 | - |
? | |
| 7E,22-ergostadien-3beta-ol + O2 | - |
Brevibacterium sterolicum | 7E,22-ergostadien-3-one + H2O2 | - |
? | |
| beta-sitosterol + O2 | - |
Nocardia erythropolis | stigmast-5-en-3-one + H2O2 | - |
? | |
| beta-sitosterol + O2 | - |
Streptomyces violascens | stigmast-5-en-3-one + H2O2 | - |
? | |
| beta-sitosterol + O2 | - |
Brevibacterium sterolicum | stigmast-5-en-3-one + H2O2 | - |
? | |
| campesterol + O2 | - |
Streptomyces violascens | (24R)-ergost-5-en-3-one + H2O2 | - |
? | |
| campesterol + O2 | - |
Brevibacterium sterolicum | (24R)-ergost-5-en-3-one + H2O2 | - |
? | |
| campesterol + O2 | 67% of activity with cholesterol | Nocardia erythropolis | (24R)-ergost-5-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | overview: substrate specificity | Nocardia erythropolis | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | overview: substrate specificity | Streptomyces violascens | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | overview: substrate specificity | Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | first step in cholesterol degradation | Nocardia erythropolis | cholest-5-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | first step in cholesterol degradation | Streptomyces violascens | cholest-5-en-3-one + H2O2 | - |
? | |
| cholesterol + O2 | first step in cholesterol degradation | Brevibacterium sterolicum | cholest-5-en-3-one + H2O2 | - |
? | |
| desmosterol + O2 | - |
Streptomyces violascens | cholesta-5,24-dien-3-one + H2O2 | - |
? | |
| desmosterol + O2 | - |
Brevibacterium sterolicum | cholesta-5,24-dien-3-one + H2O2 | - |
? | |
| desmosterol + O2 | 98% of activity with cholesteol | Nocardia erythropolis | cholesta-5,24-dien-3-one + H2O2 | - |
? | |
| ergosterol + O2 | - |
Nocardia erythropolis | dehydroergosterol + H2O2 | - |
? | |
| ergosterol + O2 | - |
Streptomyces violascens | dehydroergosterol + H2O2 | - |
? | |
| ergosterol + O2 | - |
Brevibacterium sterolicum | dehydroergosterol + H2O2 | - |
? | |
| fucosterol + O2 | - |
Nocardia erythropolis | stigmasta-5,24(28)-dien-3-one + H2O2 | - |
? | |
| stigmasterol + O2 | - |
Nocardia erythropolis | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| stigmasterol + O2 | - |
Streptomyces violascens | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
| stigmasterol + O2 | - |
Brevibacterium sterolicum | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
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