Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Nocardia sp. | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Nocardia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
polyethylene glycol derivative-modified enzyme | Nocardia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-sitosterol + O2 | - |
Nocardia sp. | stigmast-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Nocardia sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Nocardia sp. | cholest-5-en-3-one + H2O2 | - |
? | |
fucosterol + O2 | - |
Nocardia sp. | stigmasta-5,24(28)-dien-3-one + H2O2 | - |
? |