BRENDA - Enzyme Database
show all sequences of 1.1.3.6

Some enzymatic properties of 3beta-hydroxysteroid oxidase produced by Streptomyces violascens

Tomioka, H.; Kagawa, M.; Nakamura, S.; J. Biochem. 79, 903-915 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1-Fluoro-2,4-dinitrobenzene
0.1 mM, 71% inhibition
Streptomyces violascens
5alpha-Cholestan-3-one
-
Streptomyces violascens
5alpha-Cholestan-3beta,5alpha-diol
-
Streptomyces violascens
5alpha-Cholestan-3beta-ol
-
Streptomyces violascens
5alpha-Lanosta-8,24-dien-3beta-ol
-
Streptomyces violascens
5beta-Cholestan-3beta-ol
-
Streptomyces violascens
AgNO3
-
Streptomyces violascens
CuSO4
slight inhibition
Streptomyces violascens
FeCl3
1.7 mM, 95% inhibition
Streptomyces violascens
FeSO4
1.7 mM, 71% inhibition
Streptomyces violascens
HgCl2
-
Streptomyces violascens
Iodine
0.1 mM, 91% inhibition
Streptomyces violascens
KCN
-
Streptomyces violascens
additional information
not inhibited by p-chloromercuribenzoate; overview: inhibition by steroids
Streptomyces violascens
N-bromosuccinimide
0.01 mM, 100% inhibition
Streptomyces violascens
NaN3
-
Streptomyces violascens
Trinitrobenzene sulfonate
0.3 mM, 66% inhibition
Streptomyces violascens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Streptomyces violascens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + O2
Streptomyces violascens
-
cholest-5-en-3-one + H2O2
-
Streptomyces violascens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces violascens
-
-
-
Reaction
Reaction
Commentary
Organism
cholesterol + O2 = cholest-5-en-3-one + H2O2
bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid
Streptomyces violascens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Streptomyces violascens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
19.7
-
-
Streptomyces violascens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + O2
-
287648
Streptomyces violascens
cholest-4-en-3-one + H2O2
-
287648
Streptomyces violascens
?
cholesterol + O2
-
287648
Streptomyces violascens
cholest-5-en-3-one + H2O2
-
287648
Streptomyces violascens
?
dehydroepiandrosterone + O2
-
287648
Streptomyces violascens
androst-5-en-3,17-dione + H2O2
-
287648
Streptomyces violascens
-
pregnenolone + O2
-
287648
Streptomyces violascens
5-pregnen-3,20-dione + H2O2
-
287648
Streptomyces violascens
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Streptomyces violascens
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
80
about 25% of maximal activity at 10°C and 80°C
Streptomyces violascens
20
70
about 50% of maximal activity at 20°C and 70°C
Streptomyces violascens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces violascens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-Fluoro-2,4-dinitrobenzene
0.1 mM, 71% inhibition
Streptomyces violascens
5alpha-Cholestan-3-one
-
Streptomyces violascens
5alpha-Cholestan-3beta,5alpha-diol
-
Streptomyces violascens
5alpha-Cholestan-3beta-ol
-
Streptomyces violascens
5alpha-Lanosta-8,24-dien-3beta-ol
-
Streptomyces violascens
5beta-Cholestan-3beta-ol
-
Streptomyces violascens
AgNO3
-
Streptomyces violascens
CuSO4
slight inhibition
Streptomyces violascens
FeCl3
1.7 mM, 95% inhibition
Streptomyces violascens
FeSO4
1.7 mM, 71% inhibition
Streptomyces violascens
HgCl2
-
Streptomyces violascens
Iodine
0.1 mM, 91% inhibition
Streptomyces violascens
KCN
-
Streptomyces violascens
additional information
not inhibited by p-chloromercuribenzoate; overview: inhibition by steroids
Streptomyces violascens
N-bromosuccinimide
0.01 mM, 100% inhibition
Streptomyces violascens
NaN3
-
Streptomyces violascens
Trinitrobenzene sulfonate
0.3 mM, 66% inhibition
Streptomyces violascens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel filtration
Streptomyces violascens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + O2
Streptomyces violascens
-
cholest-5-en-3-one + H2O2
-
Streptomyces violascens
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Streptomyces violascens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
19.7
-
-
Streptomyces violascens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + O2
-
287648
Streptomyces violascens
cholest-4-en-3-one + H2O2
-
287648
Streptomyces violascens
?
cholesterol + O2
-
287648
Streptomyces violascens
cholest-5-en-3-one + H2O2
-
287648
Streptomyces violascens
?
dehydroepiandrosterone + O2
-
287648
Streptomyces violascens
androst-5-en-3,17-dione + H2O2
-
287648
Streptomyces violascens
-
pregnenolone + O2
-
287648
Streptomyces violascens
5-pregnen-3,20-dione + H2O2
-
287648
Streptomyces violascens
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Streptomyces violascens
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
80
about 25% of maximal activity at 10°C and 80°C
Streptomyces violascens
20
70
about 50% of maximal activity at 20°C and 70°C
Streptomyces violascens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces violascens
Other publictions for EC 1.1.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741650
Kumari
Purification and characteriza ...
Bacillus subtilis
Appl. Biochem. Biotechnol.
178
353-367
2016
1
-
-
-
-
-
-
2
1
-
1
1
-
5
-
-
1
-
-
-
1
-
2
1
1
1
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
1
1
-
-
-
1
-
-
1
-
2
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
742201
Yehia
Purification and characterisa ...
Enterococcus hirae, Enterococcus hirae Mil-31
BMC Microbiol.
15
178
2015
-
-
-
-
-
-
5
-
1
-
-
-
-
3
-
-
1
-
-
-
1
2
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
1
2
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
742709
Kaur
-
Production and optimization o ...
Rhodococcus sp., Rhodococcus sp. COP 2
Int. J. Pharm. Pharm. Sci.
7
265-268
2015
-
-
-
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
1
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
742934
Doukyu
Cholesterol oxidase with high ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa PA157
J. Biosci. Bioeng.
120
24-30
2015
6
-
1
-
-
-
3
1
1
1
-
2
8
4
-
-
-
-
-
-
-
-
11
1
1
-
3
-
1
-
3
1
-
1
-
6
-
1
1
-
-
-
-
3
-
1
1
1
-
2
8
-
-
-
-
-
-
-
11
1
1
-
3
-
1
-
3
1
-
-
-
-
-
-
742970
Shao
-
Bioconversion of cholesterol ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12
J. Chem. Technol. Biotechnol.
90
1811-1820
2015
-
-
1
-
-
-
7
3
2
3
-
4
-
4
-
-
1
-
-
-
4
-
8
1
1
-
-
-
1
-
-
1
-
-
-
-
-
2
2
-
-
-
-
14
-
4
4
6
-
4
-
-
-
2
-
-
6
-
8
2
2
-
-
-
2
-
-
-
-
1
2
-
-
-
743585
Pathak
Artificial intelligence versu ...
Streptomyces sp. NCIM 5500
PLoS ONE
10
e0137268
2015
-
-
-
-
-
-
-
-
1
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
743737
Kasabe
Assessment of alkaline choles ...
Rhodococcus sp., Rhodococcus sp. PKPD-CL
Protein Expr. Purif.
116
30-41
2015
6
-
-
-
-
1
17
1
1
1
-
2
9
4
-
-
1
-
-
-
1
2
12
1
1
1
2
-
1
1
3
1
-
1
-
6
-
-
1
-
-
1
-
17
-
1
1
1
-
2
9
-
-
1
-
-
1
2
12
1
1
1
2
-
1
1
3
1
-
1
1
-
-
-
741512
Golden
High-resolution structures of ...
Streptomyces sp. SA-COO
Acta Crystallogr. Sect. D
70
3155-3166
2014
-
-
1
1
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
742195
Reiss
Cloning, expression and bioch ...
Chryseobacterium gleum, Chryseobacterium gleum DSM 16776
BMC Biotechnol.
14
46
2014
-
-
1
-
-
-
-
2
1
-
1
2
-
4
-
-
1
-
-
-
1
-
8
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
1
-
1
2
-
-
-
1
-
-
1
-
8
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
742238
Liu
Cholesterol oxidase from Bord ...
Bordetella sp.
Cell Death Dis.
5
e1372
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
742798
Niwas
Cholesterol oxidase productio ...
Streptomyces sp.
J. Basic Microbiol.
54
1233-1239
2014
-
-
-
-
-
-
-
2
1
-
-
1
-
3
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725743
Kojima
-
Mutational analysis of the oxy ...
Streptomyces sp.
J. Mol. Catal. B
88
41-46
2013
-
1
1
-
9
-
1
2
-
-
-
1
-
1
-
-
1
-
-
-
7
-
3
-
1
-
-
2
1
-
-
1
-
-
-
-
1
1
1
-
9
-
-
1
-
2
-
-
-
1
-
-
-
1
-
-
7
-
3
-
1
-
-
2
1
-
-
-
-
-
-
-
2
2
725869
Yao
Identification and engineering ...
Mycolicibacterium neoaurum
Metab. Eng.
15
75-87
2013
-
-
1
-
1
-
-
14
2
-
3
14
-
4
-
-
-
-
-
-
14
-
15
1
1
-
-
-
1
-
-
1
-
-
-
-
-
2
2
-
2
-
-
-
-
15
4
-
3
14
-
-
-
-
-
-
15
-
15
2
2
-
-
-
2
-
-
-
-
3
6
-
-
-
726533
Niwas
Production, purification and c ...
Streptomyces sp., Streptomyces sp. R-6
World J. Microbiol. Biotechnol.
29
2077-2085
2013
6
-
-
-
-
-
10
1
1
3
1
2
1
6
-
-
1
-
-
1
1
-
2
1
1
1
-
-
1
1
-
-
-
-
-
6
-
-
-
-
-
-
-
10
-
1
1
3
1
2
1
-
-
1
-
1
1
-
2
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
743637
Klink
Cholesterol oxidase is indisp ...
Mycobacterium tuberculosis
PLoS ONE
8
e73333
2013
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
712958
Fernandez de Las Heras
ChoG is the main inducible ext ...
Rhodococcus sp., Rhodococcus sp. CECT 3014
Microbiol. Res.
166
403-418
2011
-
-
-
-
-
-
-
-
1
-
1
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
723899
Xin
-
Expression and comparison of r ...
Brevibacterium sp., Brevibacterium sp. M201008
Afr. J. Biotechnol.
10
14968-14976
2011
-
-
1
-
-
-
-
4
-
-
-
2
-
2
-
-
-
-
-
-
4
-
2
1
1
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
4
-
2
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
723968
Praveen
Purification and characterizat ...
Streptomyces parvus 13647 J, Streptomyces parvus
Appl. Biochem. Biotechnol.
165
1414-1426
2011
-
1
-
-
-
-
6
-
1
1
1
2
-
4
-
-
1
-
-
1
1
-
14
1
1
-
5
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
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6
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1
1
1
2
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1
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1
1
-
14
1
1
-
5
-
1
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-
-
710910
Saxena
Silk mat as bio-matrix for the ...
Pseudomonas fluorescens
Appl. Biochem. Biotechnol.
162
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2010
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1
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1
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1
1
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-
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1
1
710964
Li
Cholesterol oxidase ChoL is a ...
Streptomyces virginiae, Streptomyces virginiae IBL-14
Appl. Microbiol. Biotechnol.
85
1831-1838
2010
-
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1
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4
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1
1
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4
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2
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1
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8
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1
1
1
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1
1
-
1
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-
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-
-
711588
Volonte
Production of recombinant chol ...
Brevibacterium sterolicum
BMC Biotechnol.
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33
2010
-
1
1
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4
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1
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712891
Sagermann
Structural characterization of ...
Chromobacterium sp.
J. Struct. Biol.
170
32-40
2010
-
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1
1
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3
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1
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1
1
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1
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1
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1
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-
-
-
-
-
-
726408
Lin
-
Purification and characterizat ...
Bordetella sp., Bordetella sp. B4
Process Biochem.
45
1563-1569
2010
-
-
-
-
-
-
3
1
1
11
1
2
10
2
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1
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1
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2
1
1
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3
-
1
1
3
-
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3
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1
1
11
1
2
10
-
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1
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-
1
-
2
1
1
-
3
-
1
1
3
-
-
-
-
-
-
-
697914
Vrielink
Cholesterol oxidase: biochemis ...
Brevibacterium sterolicum, Streptomyces hygroscopicus
FEBS J.
276
6826-6843
2009
-
-
-
-
-
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22
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2
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10
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23
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2
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2
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22
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10
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23
-
-
-
-
-
-
-
-
-
-
697915
Pollegioni
Cholesterol oxidase: biotechno ...
Arthrobacter sp., Brevibacterium sterolicum, Mycobacterium sp., Nocardia erythropolis, Nocardia rhodochrous, Pseudomonas spp., Rhodococcus hoagii, Schizophyllum commune, Streptomyces violascens, Streptoverticillum cholesterolicum, uncultured gamma proteobacterium, uncultured gamma proteobacterium Y-134
FEBS J.
276
6857-6870
2009
-
33
-
-
3
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15
-
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15
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11
-
37
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-
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11
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-
33
-
11
-
3
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15
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11
-
37
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-
-
-
-
-
-
-
-
710711
Lyubimov
A hydrogen-bonding network is ...
Streptomyces sp.
Acta Crystallogr. Sect. D
65
1222-1231
2009
-
-
1
1
2
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6
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1
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1
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1
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6
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1
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1
1
1
2
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6
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1
-
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-
1
-
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-
-
6
-
-
-
-
-
-
-
-
-
-
710959
Doukyu
Characteristics and biotechnol ...
Arthrobacter sp., Arthrobacter sp. IM79, Brevibacterium sterolicum, Burkholderia cepacia, Burkholderia cepacia ST-200, Burkholderia pseudomallei, Burkholderia thailandensis, Chromobacterium sp., Corynebacterium cholesterolicum, Corynebacterium urealyticum, Corynebacterium urealyticum DSM 7109, Mycobacterium leprae, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Nocardia farcinica, Nocardia farcinica IFM 10152, Nocardia rhodochrous, Nocardia sp., Paraburkholderia xenovorans, Pimelobacter simplex, Pseudomonas aeruginosa, Pseudomonas sp., Pseudomonas sp. COX629, Rhodococcus erythropolis, Rhodococcus hoagii 103S, Rhodococcus hoagii, Schizophyllum commune, Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces fradiae, Streptomyces griseus, Streptomyces natalensis, Streptomyces sp., Streptomyces violascens, Streptoverticillium cholesterolieum, uncultured gamma proteobacterium, uncultured gamma proteobacterium Y-134
Appl. Microbiol. Biotechnol.
83
825-837
2009
-
-
-
-
-
-
26
3
-
3
13
110
24
44
-
-
-
-
-
1
-
-
110
-
11
-
6
-
17
-
9
29
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-
-
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-
-
31
-
-
-
-
26
-
3
-
3
13
110
24
-
-
-
-
1
-
-
110
-
11
-
6
-
17
-
9
-
-
2
2
-
-
-
713589
Ghasemian
-
Overexpression, one-step purif ...
Rhodococcus sp., Rhodococcus sp. PTCC 1633
World J. Microbiol. Biotechnol.
25
773-779
2009
-
-
1
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1
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1
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2
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1
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8
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1
-
1
-
1
1
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1
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1
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1
1
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-
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-
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1
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1
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1
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8
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
684653
Doukyu
Purification and characterizat ...
Chromobacterium sp. DS-1
Appl. Microbiol. Biotechnol.
80
59-70
2008
-
-
-
-
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1
1
1
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12
3
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1
-
-
1
1
-
9
-
1
1
5
-
1
1
1
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
-
-
12
-
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1
-
1
1
-
9
-
1
1
5
-
1
1
1
-
-
-
-
-
-
-
685245
Chen
The binding and release of oxy ...
Streptomyces sp.
Biochemistry
47
5368-5377
2008
-
-
-
1
3
-
1
8
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-
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2
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1
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1
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8
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2
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1
3
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1
2
8
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-
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1
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-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
685754
Park
Heterologous expression of cho ...
Streptomyces coelicolor
Biotechnol. Lett.
30
165-172
2008
-
-
1
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-
-
-
-
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4
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1
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-
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-
-
-
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-
-
-
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-
-
-
-
-
687754
Piubelli
On the oxygen reactivity of fl ...
Brevibacterium sterolicum
J. Biol. Chem.
283
24738-24747
2008
-
-
1
-
3
-
-
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2
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1
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1
1
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3
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687977
Srisawasdi
Implementation of cellulomonas ...
Cellulomonas sp.
J. Clin. Lab. Anal.
22
50-58
2008
-
1
-
-
-
-
-
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3
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2
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2
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-
-
-
-
-
-
-
-
688918
Aparicio
Microbial cholesterol oxidases ...
Arthrobacter sp., Brevibacterium sterolicum, Rhodococcus erythropolis, Rhodococcus hoagii, Rhodococcus rhodochrous, Schizophyllum commune, Streptomyces sp.
Mol. Biosyst.
4
804-809
2008
-
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-
2
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-
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7
-
-
1
-
9
-
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5
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8
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2
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7
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1
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5
-
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8
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
689918
Caldinelli
Relevance of the flavin bindin ...
Brevibacterium sterolicum
Protein Sci.
17
409-419
2008
-
-
-
-
1
-
-
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1
2
-
-
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-
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1
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1
-
1
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1
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-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
677549
Singh
Immobilization of cholesterol ...
Pseudomonas sp.
Anal. Chim. Acta
582
335-343
2007
-
1
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-
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2
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1
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1
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1
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-
-
-
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-
-
-
-
684242
Mahmoud
-
Extracellular Pleurotus ostrea ...
Pleurotus ostreatus
Adv. Food Sci.
29
12-20
2007
-
-
-
-
-
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4
1
1
-
1
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-
1
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1
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1
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1
1
1
-
1
-
1
-
1
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-
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4
-
1
1
-
1
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1
-
1
-
-
1
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
684447
Hejazi
-
Cloning and sequencing of part ...
Streptomyces luridus
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57
259-263
2007
-
-
1
-
-
-
-
-
-
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1
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1
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1
1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
685744
Wang
Coenzyme precursor-assisted ex ...
Brevibacterium sp. DGCDC-82, Brevibacterium sp. DGCDC-82 CCTCC M201008
Biotechnol. Lett.
29
761-766
2007
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1
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6
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1
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686044
Nesbitt
Antifungal tradecraft by chole ...
Streptomyces natalensis
Chem. Biol.
14
238-241
2007
-
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-
-
-
-
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1
-
1
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2
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1
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1
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2
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686045
Mendes
Cholesterol oxidases act as si ...
Streptomyces natalensis
Chem. Biol.
14
279-290
2007
-
-
-
-
-
-
-
-
1
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1
1
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2
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1
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1
1
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3
1
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1
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1
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1
-
1
1
-
3
1
-
-
-
-
-
-
-
-
-
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-
-
-
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686440
Oezer
-
Amperometric enzyme electrode ...
Streptomyces sp.
Enzyme Microb. Technol.
40
262-265
2007
-
1
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-
-
-
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1
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1
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686792
Brzostek
Cholesterol oxidase is require ...
Mycobacterium tuberculosis
FEMS Microbiol. Lett.
275
106-112
2007
-
-
-
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-
-
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5
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1
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1
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1
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-
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688610
Bhattacharyya
Combined quantum mechanical an ...
Streptomyces sp.
J. Phys. Chem. A
111
5729-5742
2007
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1
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1
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1
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689910
Lyubimov
Distortion of flavin geometry ...
Streptomyces sp.
Protein Sci.
16
2647-2656
2007
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1
1
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-
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1
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1
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1
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1
1
1
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1
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-
-
690066
Parra
-
Cholesterol oxidase modified g ...
Streptomyces sp.
Sens. Actuators B Chem.
B124
30-37
2007
-
1
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1
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1
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-
-
-
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-
672460
Basu
Development of cholesterol bio ...
Brevibacterium sterolicum
Bioelectrochemistry
70
375-379
2006
-
1
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1
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Expression of a cholesterol ox ...
Pimelobacter simplex, Pimelobacter simplex F2
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Dissecting the structural dete ...
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654191
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Steady-state oxidation of chol ...
Pseudomonas sp.
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655113
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Cholesterol oxidation using ho ...
Pseudomonas sp.
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Purification and some properti ...
gamma proteobacterium Y-134
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7
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656321
Isobe
The second cholesterol oxidase ...
gamma proteobacterium Y-134
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7
1
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Sub-atomic resolution crystal ...
Streptomyces sp.
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Aunpad
Crystallization and preliminar ...
Burkholderia cepacia
Acta Crystallogr. Sect. D
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2002
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Yin
Construction of a catalyticall ...
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6
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Rhee
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Two novel extracellular choles ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) SFF34
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25
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25
1
2
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2
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655128
Sojo
Rhodococcus erythropolis ATCC ...
Rhodococcus erythropolis
BMC Biotechnol.
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Fujishiro
Purification and properties of ...
Brevibacterium sterolicum
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2002
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9
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2
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9
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Alteration of substrate specif ...
Streptomyces sp.
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2002
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1
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9
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19
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1
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1
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19
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287676
Motteront
Cholesterol oxidase from Brevi ...
Brevibacterium sterolicum
J. Biol. Chem.
276
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2001
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1
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1
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2
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3
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1
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1
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1
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1
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1
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2
-
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-
3
-
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-
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-
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-
-
-
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287677
Doukyu
Cloning, sequence analysis and ...
Brevibacterium sterolicum, Burkholderia cepacia, Burkholderia cepacia ST-200, Nocardia erythropolis, Pseudomonas sp., Streptomyces sp.
Appl. Microbiol. Biotechnol.
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2001
2
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1
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10
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14
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2
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10
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6
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1
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1
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14
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1
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-
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287675
MacLachlan
Cholesterol oxidase: sources, ...
Brevibacterium sterolicum, Comamonas testosteroni, Corynebacterium cholesterolicum, Mycobacterium sp., Nocardia erythropolis, Nocardia rhodochrous, Nocardia sp., Pimelobacter simplex, Pseudomonas sp., Rhodococcus erythropolis, Rhodococcus hoagii, Rhodococcus sp., Rhodococcus sp. GK1, Schizophyllum commune, Streptomyces griseocarneus, Streptomyces hygroscopicus, Streptomyces lavendulae, Streptomyces lividans, Streptomyces sp., Streptomyces violascens
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1
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2
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18
1
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4
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1
20
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3
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40
1
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2
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287672
Kimberley Yue
Crystal structure determinatio ...
Streptomyces sp.
Biochemistry
38
4277-4286
1999
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2
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1
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1
3
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2
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1
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3
-
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-
-
2
-
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-
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-
-
-
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287673
Pollegioni
Cholesterol oxidase from Strep ...
Brevibacterium sterolicum, Streptomyces hygroscopicus
Biotechnol. Appl. Biochem.
30
27-33
1999
5
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2
2
1
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2
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2
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4
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5
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2
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2
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1
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2
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4
-
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-
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287674
Pollegioni
Kinetic mechanisms of choleste ...
Brevibacterium sterolicum, Streptomyces hygroscopicus
Eur. J. Biochem.
264
140-151
1999
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2
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12
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17
-
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2
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2
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14
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2
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12
-
-
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17
-
-
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287679
Doukyu
Purification of extracellular ...
Pseudomonas sp., Pseudomonas sp. ST-200
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1998
5
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1
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4
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1
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1
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1
1
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5
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1
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2
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1
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1
1
-
4
-
1
-
1
-
1
1
-
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-
-
-
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287669
Nishiya
Improvement of thermal stabili ...
Streptomyces sp.
Protein Eng.
10
231-235
1997
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1
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4
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3
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1
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2
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1
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8
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2
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1
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1
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2
1
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1
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4
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3
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1
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8
-
2
-
1
-
1
-
2
1
-
-
-
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-
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-
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287670
Gadda
Characterization of cholestero ...
Brevibacterium sterolicum, Streptomyces hygroscopicus
Eur. J. Biochem.
250
369-376
1997
2
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1
12
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4
2
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5
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2
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7
2
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12
-
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2
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2
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2
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1
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12
-
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4
2
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-
-
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7
2
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-
-
12
-
-
-
-
-
-
-
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287671
Sojo
Cell-linked and extracellular ...
Nocardia rhodochrous, Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
47
583-589
1997
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1
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1
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2
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1
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4
-
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287678
Ghoshroy
Investigation of membrane disr ...
Streptomyces sp., Streptomyces sp. A19249
Biochemistry
36
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1997
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1
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4
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287668
Croteau
Crystallization and preliminar ...
Brevibacterium sterolicum
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317-319
1996
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1
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1
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1
1
1
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1
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2
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287667
Li
Crystal structure of cholester ...
Brevibacterium sterolicum
Biochemistry
32
11507-11515
1993
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1
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2
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1
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2
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1
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1
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1
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2
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-
-
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287662
Johnson
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Isolation of cholesterol oxida ...
Rhodococcus hoagii
Biotechnol. Appl. Biochem.
13
196-204
1991
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1
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2
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1
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2
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1
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2
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287659
Lee
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Purification and characterizat ...
Pseudomonas sp.
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31
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3
1
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1
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7
1
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1
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1
1
2
1
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1
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3
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1
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1
1
1
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Molecular characteristics of t ...
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Purification and some properti ...
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2892
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Shirokane
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Purification and some properti ...
Corynebacterium cholesterolicum
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Tomioka
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Smith
Cholesterol oxidases: properti ...
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1976
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Smith
Studies of the substrate speci ...
Nocardia erythropolis
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Brooks
Cholesterol oxidase. Further s ...
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1975
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Kerenyi
Properties of delta5-3beta-hyd ...
Streptomyces griseocarneus
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1975
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Smith
Application of cholesterol oxi ...
Nocardia erythropolis
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Buckland
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The large scale isolation of i ...
Nocardia rhodochrous
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1974
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Uwajima
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Properties of crystalline 3bet ...
Brevibacterium sterolicum
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Fukuda
A method to screen anticholest ...
Streptomyces violascens
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Uwajima
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Isolation and crystallization ...
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Microbiol transformation of st ...
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Stadman
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