Literature summary for 1.1.3.5 extracted from

  • Groen, B.W.; de Vries, S.; Duine, J.A.
    Characterization of hexose oxidase from the red seaweed Chondrus crispus (1997), Eur. J. Biochem., 244, 858-861.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ethanol 100 mM, 1.5fold increase in activity Chondrus crispus
NaCl 20 mM, 1.4fold increase in activity Chondrus crispus

General Stability

General Stability Organism
very stable enzyme, no activity decrease after storage at room temperature overnight Chondrus crispus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
O2
-
Chondrus crispus
1.7
-
lactose
-
Chondrus crispus
12.5
-
cellobiose
-
Chondrus crispus
20
-
D-galactose
-
Chondrus crispus
8.5
-
D-glucose
-
Chondrus crispus
28
-
maltose
-
Chondrus crispus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
native PAGE on gradient gel Chondrus crispus

Organism

Organism UniProt Comment Textmining
Chondrus crispus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Chondrus crispus

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, Phenyl-sepharose, Superdex 200 Chondrus crispus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
-
Chondrus crispus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + O2
-
Chondrus crispus 4-O-(beta-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
?
D-galactose + O2
-
Chondrus crispus D-galactono-1,5-lactone + H2O2
-
?
D-glucose + O2
-
Chondrus crispus D-glucono-1,5-lactone + H2O2
-
?
lactose + O2
-
Chondrus crispus 4-O-(beta-D-galactopyranosido)-D-glucono-1,5-lactone + H2O2
-
?
maltose + O2
-
Chondrus crispus 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
broad pH range, two optima can be distinguished Chondrus crispus