Literature summary for 1.1.3.5 extracted from

  • Ikawa, M.
    Hexose oxidase from Chondrus crispus (1982), Methods Enzymol., 89, 145-149.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
acetate
-
Chondrus crispus
azide
-
Chondrus crispus
cyanide
-
Chondrus crispus
diethyldithiocarbamate
-
Chondrus crispus
hydroxylamine
-
Chondrus crispus
pyruvate
-
Chondrus crispus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8
-
D-galactose
-
Chondrus crispus
4
-
D-glucose
-
Chondrus crispus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 12 mol per mol enzyme Chondrus crispus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
gel filtration Chondrus crispus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Chondrus crispus
-
D-glucono-1,5-lactone + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Chondrus crispus
-
irish moss, red alga, red seaweed
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 70% carbohydrates, mainly galactose and xylose Chondrus crispus

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, Sephadex G-200 Chondrus crispus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.095
-
-
Chondrus crispus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + O2
-
Chondrus crispus D-glucono-1,5-lactone + H2O2
-
?
D-galactose + O2 82% of activity with D-glucose Chondrus crispus D-galactono-1,5-lactone + H2O2
-
?
maltose + O2 40% of activity with D-glucose Chondrus crispus 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
?
cellobiose + O2 32% of activity with D-glucose Chondrus crispus 4-O-(beta-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
?
lactose + O2 22% of activity with D-glucose Chondrus crispus 4-O-(beta-D-galactopyranosido)-D-glucono-1,5-lactone + H2O2
-
?
D-glucose-6-phosphate + O2 10% of activity with D-glucose Chondrus crispus D-glucono-1,5-lactone-6-phosphate + H2O2
-
?
D-mannose + O2 8% of activity with D-glucose Chondrus crispus D-mannono-1,5-lactone + H2O2 8% of activity with D-glucose ?
2-deoxy-D-glucose + O2 8% of activity with D-glucose Chondrus crispus 2-deoxy-D-glucono-1,5-lactone + H2O2
-
?
2-deoxy-D-galactose + O2 6% of activity with D-glucose Chondrus crispus 2-deoxy-D-galactono-1,5-lactone + H2O2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Chondrus crispus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
Chondrus crispus