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Literature summary for 1.1.3.49 extracted from

  • Ishida, Y; Kuwahara, Y.; Dadashipour, M; Ina, A; Yamaguchi, T; Morita, M.; Ichiki, Y; Asano, Y
    A sacrificial millipede altruistically protects its swarm using a drone blood enzyme, mandelonitrile oxidase (2016), Sci. Rep., 6, 26998.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.49

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 1 mM, 56% residual activity Chamberlinius hualienensis
potassium cyanide 1 mM, 12.7% residual activity Chamberlinius hualienensis
Sodium azide 1 mM, 28.6% residual activity Chamberlinius hualienensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 (R,S)-mandelonitrile pH 5.0, 25°C Chamberlinius hualienensis

Metals/Ions

Metals/Ions Comment Organism Structure
ferric chloride 1 mM, 124% of initial activity Chamberlinius hualienensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Chamberlinius hualienensis
67000
-
-
 Chamberlinius hualienensis

Organism

Organism UniProt Comment Textmining
Chamberlinius hualienensis A0A173MZJ8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains six predicted N-glycosylation sites at resiudes N57, N79, N94, N149, N406, and N491 Chamberlinius hualienensis

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Chamberlinius hualienensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3E,5E)-2-hydroxydeca-3,5-dienenitrile + O2 10% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (3E,5E)-2-oxodeca-3,5-dienenitrile + H2O2
-
 ?
(E)-2-hydroxy-4-phenylbut-3-enenitrile + O2 29% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (3E)-2-oxo-4-phenylbut-3-enenitrile + H2O2
-
 ?
(R)-mandelonitrile + O2
-
 Chamberlinius hualienensis benzoyl cyanide + H2O2 enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile ?
(R,S)-mandelonitrile + O2
-
 Chamberlinius hualienensis benzoyl cyanide + H2O2
-
 ?
2-(3-bromophenyl)-2-hydroxyacetonitrile + O2 22% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (3-bromophenyl)(oxo)acetonitrile + H2O2
-
 ?
2-hydroxy-2-(2-methoxyphenyl)acetonitrile + O2 17% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (2-methoxyphenyl)(oxo)acetonitrile + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE, 1 * 62818, calculated Chamberlinius hualienensis

Synonyms

Synonyms Comment Organism
ChuaMOX
-
 Chamberlinius hualienensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Chamberlinius hualienensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 90% of maximum activity Chamberlinius hualienensis
40
-
 90% of maximum activity Chamberlinius hualienensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 1 h, complete loss of activity Chamberlinius hualienensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
 Chamberlinius hualienensis

pH Range

pH Minimum pH Maximum Comment Organism
3.5
-
 80% of maximum activity Chamberlinius hualienensis
5
-
 80% of maximum activity Chamberlinius hualienensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 25-50 °C, 1 h, 80% residual activity Chamberlinius hualienensis

Cofactor

Cofactor Comment Organism Structure
FAD sequence contains a FAD binding motif in the T22-S62 region Chamberlinius hualienensis

pI Value

Organism Comment pI Value Maximum pI Value
Chamberlinius hualienensis calculated
-
 6.3

General Information

General Information Comment Organism
physiological function the enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile. The enzymatic activity is suppressed at a blood pH of 7, and the enzyme is segregated by membranes of defensive sacs from its substrate mandelonitrile which has a pH of 4.6, the optimum pH for ChuaMOX activity. Strong body muscle contractions are necessary for de novo synthesis of benzoyl cyanide Chamberlinius hualienensis