BRENDA - Enzyme Database
show all sequences of 1.1.3.49

A sacrificial millipede altruistically protects its swarm using a drone blood enzyme, mandelonitrile oxidase

Ishida, Y; Kuwahara, Y.; Dadashipour, M; Ina, A; Yamaguchi, T; Morita, M.; Ichiki, Y; Asano, Y; Sci. Rep. 6, 26998 (2016)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 56% residual activity
Chamberlinius hualienensis
potassium cyanide
1 mM, 12.7% residual activity
Chamberlinius hualienensis
Sodium azide
1 mM, 28.6% residual activity
Chamberlinius hualienensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1
-
(R,S)-mandelonitrile
pH 5.0, 25C
Chamberlinius hualienensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
ferric chloride
1 mM, 124% of initial activity
Chamberlinius hualienensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
gel filtration
Chamberlinius hualienensis
67000
-
-
Chamberlinius hualienensis
Organism
Organism
UniProt
Commentary
Textmining
Chamberlinius hualienensis
A0A173MZJ8
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
sequence contains six predicted N-glycosylation sites at resiudes N57, N79, N94, N149, N406, and N491
Chamberlinius hualienensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
blood
-
Chamberlinius hualienensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(3E,5E)-2-hydroxydeca-3,5-dienenitrile + O2
10% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(3E,5E)-2-oxodeca-3,5-dienenitrile + H2O2
-
-
-
?
(E)-2-hydroxy-4-phenylbut-3-enenitrile + O2
29% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(3E)-2-oxo-4-phenylbut-3-enenitrile + H2O2
-
-
-
?
(R)-mandelonitrile + O2
-
739691
Chamberlinius hualienensis
benzoyl cyanide + H2O2
enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile
-
-
?
(R,S)-mandelonitrile + O2
-
739691
Chamberlinius hualienensis
benzoyl cyanide + H2O2
-
-
-
?
2-(3-bromophenyl)-2-hydroxyacetonitrile + O2
22% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(3-bromophenyl)(oxo)acetonitrile + H2O2
-
-
-
?
2-hydroxy-2-(2-methoxyphenyl)acetonitrile + O2
17% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(2-methoxyphenyl)(oxo)acetonitrile + H2O2
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 67000, SDS-PAGE, 1 * 62818, calculated
Chamberlinius hualienensis
Synonyms
Synonyms
Commentary
Organism
ChuaMOX
-
Chamberlinius hualienensis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Chamberlinius hualienensis
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
-
90% of maximum activity
Chamberlinius hualienensis
40
-
90% of maximum activity
Chamberlinius hualienensis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
70
-
1 h, complete loss of activity
Chamberlinius hualienensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
4.5
-
Chamberlinius hualienensis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.5
-
80% of maximum activity
Chamberlinius hualienensis
5
-
80% of maximum activity
Chamberlinius hualienensis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
25-50 C, 1 h, 80% residual activity
Chamberlinius hualienensis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
sequence contains a FAD binding motif in the T22-S62 region
Chamberlinius hualienensis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Chamberlinius hualienensis
calculated
-
6.3
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
sequence contains a FAD binding motif in the T22-S62 region
Chamberlinius hualienensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 56% residual activity
Chamberlinius hualienensis
potassium cyanide
1 mM, 12.7% residual activity
Chamberlinius hualienensis
Sodium azide
1 mM, 28.6% residual activity
Chamberlinius hualienensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1
-
(R,S)-mandelonitrile
pH 5.0, 25C
Chamberlinius hualienensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
ferric chloride
1 mM, 124% of initial activity
Chamberlinius hualienensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
65000
-
gel filtration
Chamberlinius hualienensis
67000
-
-
Chamberlinius hualienensis
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
sequence contains six predicted N-glycosylation sites at resiudes N57, N79, N94, N149, N406, and N491
Chamberlinius hualienensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
blood
-
Chamberlinius hualienensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(3E,5E)-2-hydroxydeca-3,5-dienenitrile + O2
10% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(3E,5E)-2-oxodeca-3,5-dienenitrile + H2O2
-
-
-
?
(E)-2-hydroxy-4-phenylbut-3-enenitrile + O2
29% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(3E)-2-oxo-4-phenylbut-3-enenitrile + H2O2
-
-
-
?
(R)-mandelonitrile + O2
-
739691
Chamberlinius hualienensis
benzoyl cyanide + H2O2
enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile
-
-
?
(R,S)-mandelonitrile + O2
-
739691
Chamberlinius hualienensis
benzoyl cyanide + H2O2
-
-
-
?
2-(3-bromophenyl)-2-hydroxyacetonitrile + O2
22% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(3-bromophenyl)(oxo)acetonitrile + H2O2
-
-
-
?
2-hydroxy-2-(2-methoxyphenyl)acetonitrile + O2
17% of the activity with (R)-mandelonitrile
739691
Chamberlinius hualienensis
(2-methoxyphenyl)(oxo)acetonitrile + H2O2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 67000, SDS-PAGE, 1 * 62818, calculated
Chamberlinius hualienensis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Chamberlinius hualienensis
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
-
90% of maximum activity
Chamberlinius hualienensis
40
-
90% of maximum activity
Chamberlinius hualienensis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
70
-
1 h, complete loss of activity
Chamberlinius hualienensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
4.5
-
Chamberlinius hualienensis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.5
-
80% of maximum activity
Chamberlinius hualienensis
5
-
80% of maximum activity
Chamberlinius hualienensis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
25-50 C, 1 h, 80% residual activity
Chamberlinius hualienensis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Chamberlinius hualienensis
calculated
-
6.3
General Information
General Information
Commentary
Organism
physiological function
the enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile. The enzymatic activity is suppressed at a blood pH of 7, and the enzyme is segregated by membranes of defensive sacs from its substrate mandelonitrile which has a pH of 4.6, the optimum pH for ChuaMOX activity. Strong body muscle contractions are necessary for de novo synthesis of benzoyl cyanide
Chamberlinius hualienensis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile. The enzymatic activity is suppressed at a blood pH of 7, and the enzyme is segregated by membranes of defensive sacs from its substrate mandelonitrile which has a pH of 4.6, the optimum pH for ChuaMOX activity. Strong body muscle contractions are necessary for de novo synthesis of benzoyl cyanide
Chamberlinius hualienensis
Other publictions for EC 1.1.3.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739691
Ishida
A sacrificial millipede altrui ...
Chamberlinius hualienensis
Sci. Rep.
6
26998
2016
-
-
-
-
-
-
3
1
-
1
2
-
-
6
-
1
-
-
-
1
-
-
6
1
1
1
2
1
-
1
2
1
1
-
1
-
-
-
-
1
-
-
-
-
3
-
1
-
1
2
-
-
-
1
-
-
1
-
-
6
1
1
2
1
-
1
2
1
1
-
1
1
-
-
-