BRENDA - Enzyme Database show
show all sequences of 1.1.3.48

Structures of KdnB and KdnA from Shewanella oneidensis: Key enzymes in the formation of 8-amino-3,8-dideoxy-D-manno-octulosonic acid

Zachman-Brockmeyer, T.R.; Thoden, J.B.; Holden, H.M.; Biochemistry 55, 4485-4494 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta2-(DE3) cells
Shewanella oneidensis
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 7-9% (w/v) poly(ethylene glycol) 8000, 100 mM homo-PIPES (pH 5.0), and 1.0 M tetramethylammonium chloride
Shewanella oneidensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-deoxy-alpha-D-manno-octulopyranosonate + O2
Shewanella oneidensis
-
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
?
3-deoxy-alpha-D-manno-octulopyranosonate + O2
Shewanella oneidensis ATCC 700550
-
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Shewanella oneidensis
Q8EEB0
-
-
Shewanella oneidensis ATCC 700550
Q8EEB0
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA resin column chromatography
Shewanella oneidensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-deoxy-alpha-D-manno-octulopyranosonate + O2
-
737728
Shewanella oneidensis
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
-
?
3-deoxy-alpha-D-manno-octulopyranosonate + O2
-
737728
Shewanella oneidensis ATCC 700550
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme does not require NAD(P) for activity
Shewanella oneidensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta2-(DE3) cells
Shewanella oneidensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme does not require NAD(P) for activity
Shewanella oneidensis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 7-9% (w/v) poly(ethylene glycol) 8000, 100 mM homo-PIPES (pH 5.0), and 1.0 M tetramethylammonium chloride
Shewanella oneidensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-deoxy-alpha-D-manno-octulopyranosonate + O2
Shewanella oneidensis
-
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
?
3-deoxy-alpha-D-manno-octulopyranosonate + O2
Shewanella oneidensis ATCC 700550
-
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA resin column chromatography
Shewanella oneidensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-deoxy-alpha-D-manno-octulopyranosonate + O2
-
737728
Shewanella oneidensis
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
-
?
3-deoxy-alpha-D-manno-octulopyranosonate + O2
-
737728
Shewanella oneidensis ATCC 700550
3,8-dideoxy-8-oxo-alpha-D-manno-octulosonate + H2O2
-
-
-
?
Other publictions for EC 1.1.3.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737728
Zachman-Brockmeyer
Structures of KdnB and KdnA fr ...
Shewanella oneidensis, Shewanella oneidensis ATCC 700550
Biochemistry
55
4485-4494
2016
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734255
Gattis
The origin of 8-amino-3,8-dide ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
J. Biol. Chem.
288
9216-9225
2013
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