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Literature summary for 1.1.3.47 extracted from

  • Martin, C.; Ovalle Maqueo, A.; Wijma, H.; Fraaije, M.
    Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design (2018), Biotechnol. Biofuels, 11, 56 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.47

Application

Application Comment Organism
biotechnology development of a facile gene shuffling approach to rapidly combine stabilizing mutations in a one-pot reaction. This allows the identification of the optimal combination of several beneficial mutations. The approach quickly discriminates stable and active multi-site variants, making it a very useful addition to FRESCO (framework for rapid enzyme stabilization by computational libraries) method Methylovorus sp. MP688

Protein Variants

Protein Variants Comment Organism
I73V/H74Y/G356H/V367L/T414K/A419Y/A435E most thermostable variant isolated with an improvement of melting temperature of 12 degrees compared to wild-type Methylovorus sp. MP688
I73V/H74Y/G356H/V367R/T414K/A419Y/A435E/W466F introduction of mutation W466F to mutant I73V/H74Y/G356H/V367L/T414K/A419Y/A435E leads to a decrease in thermostability but improved synthesis of furan-2,5-dicarboxylate. At 25°C, an almost full conversion in 24 h can be achieved Methylovorus sp. MP688

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
 5-(hydroxymethyl)furfural mutant I73V/H74Y/G356H/V367L/T414K/A419Y/A435E, pH 8.0, 25°C Methylovorus sp. MP688
1.5 2 5-(hydroxymethyl)furfural wild-type, pH 8.0, 25°C Methylovorus sp. MP688

Organism

Organism UniProt Comment Textmining
Methylovorus sp. MP688 E4QP00
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-(hydroxymethyl)furan-2-carbaldehyde + O2
-
 Methylovorus sp. MP688 5-formylfuran-2-carboxylate + H2O2
-
 ?
5-(hydroxymethyl)furfural + O2
-
 Methylovorus sp. MP688 furan-2,5-dicarbaldehyde + H2O2
-
 ?
5-formylfuran-2-carboxylate + O2
-
 Methylovorus sp. MP688 furan-2,5-dicarboxylate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
MPQ_0130
-
 Methylovorus sp. MP688

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48.5
-
 melting temperature, wild-type Methylovorus sp. MP688
60.5
-
 melting temperature, mutant I73V/H74Y/G356H/V367L/T414K/A419Y/A435E Methylovorus sp. MP688

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.8
-
 5-(hydroxymethyl)furfural mutant I73V/H74Y/G356H/V367L/T414K/A419Y/A435E, pH 8.0, 25°C Methylovorus sp. MP688
13.7
-
 5-(hydroxymethyl)furfural wild-type, pH 8.0, 25°C Methylovorus sp. MP688

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 5-(hydroxymethyl)furfural wild-type, pH 8.0, 25°C Methylovorus sp. MP688
18.7
-
 5-(hydroxymethyl)furfural mutant I73V/H74Y/G356H/V367L/T414K/A419Y/A435E, pH 8.0, 25°C Methylovorus sp. MP688