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Literature summary for 1.1.3.47 extracted from
- Screening and evaluation of new hydroxymethylfurfural oxidases for furandicarboxylic acid production (2020), Appl. Environ. Microbiol., 86, e00842 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas nitroreducens |
| expression in Escherichia coli | Methylovorus sp. MP688 |
| expression in Escherichia coli | Pseudomonas sp. 11/12A |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas nitroreducens |  |
| 0.7 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 0.8 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas nitroreducens | |
| 4.8 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas nitroreducens | |
| 5.1 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas nitroreducens | |
| 6.8 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Methylovorus sp. MP688 | |
| 9.1 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Methylovorus sp. MP688 | |
| 21.7 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 38.9 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas sp. 11/12A | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylovorus sp. MP688 | E4QP00 | cf. EC 1.1.3.38 | - |
| Pseudomonas nitroreducens | - |
- |
- |
| Pseudomonas sp. 11/12A | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2 | - |
recombinant protein, substrate vanillyl alcohol, pH 7.0, 25°C | Pseudomonas nitroreducens |
| 2 | - |
recombinant protein, substrate vanillyl alcohol, reaction of EC 1.1.3.47, pH 7.0, 25°C | Pseudomonas sp. 11/12A |
| 3.3 | - |
recombinant protein, substrate vanillyl alcohol, reaction of EC 1.1.3.38, pH 7.0, 25°C | Methylovorus sp. MP688 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-(hydroxymethyl)furfural + O2 | - |
Pseudomonas nitroreducens | furan-2,5-dicarbaldehyde + H2O2 | - |
? | |
| 5-(hydroxymethyl)furfural + O2 | - |
Methylovorus sp. MP688 | furan-2,5-dicarbaldehyde + H2O2 | - |
? | |
| 5-(hydroxymethyl)furfural + O2 | - |
Pseudomonas sp. 11/12A | furan-2,5-dicarbaldehyde + H2O2 | - |
? | |
| 5-formylfuran-2-carboxylate + O2 | - |
Pseudomonas nitroreducens | furan-2,5-dicarboxylate + H2O2 | - |
? | |
| 5-formylfuran-2-carboxylate + O2 | - |
Methylovorus sp. MP688 | furan-2,5-dicarboxylate + H2O2 | - |
? | |
| 5-formylfuran-2-carboxylate + O2 | - |
Pseudomonas sp. 11/12A | furan-2,5-dicarboxylate + H2O2 | - |
? | |
| furan-2,5-dicarbaldehyde + O2 | - |
Pseudomonas nitroreducens | 5-formylfuran-2-carboxylate + H2O2 | - |
? | |
| furan-2,5-dicarbaldehyde + O2 | - |
Methylovorus sp. MP688 | 5-formylfuran-2-carboxylate + H2O2 | - |
? | |
| furan-2,5-dicarbaldehyde + O2 | - |
Pseudomonas sp. 11/12A | 5-formylfuran-2-carboxylate + H2O2 | - |
? | |
| vanillyl alcohol + O2 | - |
Pseudomonas nitroreducens | vanillin + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, MALDI-TOF | Pseudomonas nitroreducens |
| ? | x * 57000, MALDI-TOF | Methylovorus sp. MP688 |
| ? | x * 57000, MALDI-TOF | Pseudomonas sp. 11/12A |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HMFO | - |
Pseudomonas nitroreducens |
| HMFO | - |
Methylovorus sp. MP688 |
| HMFO | - |
Pseudomonas sp. 11/12A |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Pseudomonas sp. 11/12A |
| 40 | 45 | - |
Pseudomonas nitroreducens |
| 45 | - |
- |
Methylovorus sp. MP688 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | more than 80% of maximum activity | Methylovorus sp. MP688 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
melting temperature | Pseudomonas sp. 11/12A |
| 44 | - |
melting temperature | Pseudomonas nitroreducens |
| 48 | - |
melting temperature | Methylovorus sp. MP688 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 0.008 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas nitroreducens | |
| 0.01 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 0.017 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas nitroreducens | |
| 0.1 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Methylovorus sp. MP688 | |
| 0.24 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas nitroreducens | |
| 1 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas nitroreducens | |
| 1 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 1.38 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Methylovorus sp. MP688 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Methylovorus sp. MP688 |
| 7.5 | 8 | - |
Pseudomonas sp. 11/12A |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | more than 65% of maximum activity | Pseudomonas nitroreducens |
| 9 | - |
complete loss of activity | Pseudomonas sp. 11/12A |
| 10 | - |
5% residual activity | Methylovorus sp. MP688 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas nitroreducens | |
| FAD | - |
Methylovorus sp. MP688 | |
| FAD | - |
Pseudomonas sp. 11/12A | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Methylovorus sp. MP688 | calculated from sequence | - |
6.1 |
| Pseudomonas sp. 11/12A | calculated from sequence | - |
6.1 |
| Pseudomonas nitroreducens | calculated from sequence | - |
6.5 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Methylovorus sp. MP688 | |
| 0.0015 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas nitroreducens | |
| 0.0023 | - |
5-formylfuran-2-carboxylate | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 0.0025 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 0.003 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Pseudomonas nitroreducens | |
| 0.015 | - |
furan-2,5-dicarbaldehyde | pH 7, 25°C | Methylovorus sp. MP688 | |
| 0.047 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas nitroreducens | |
| 0.05 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Pseudomonas sp. 11/12A | |
| 0.15 | - |
5-(hydroxymethyl)furfural | pH 7, 25°C | Methylovorus sp. MP688 | |
| 1.3 | - |
Vanillyl alcohol | pH 7, 25°C | Pseudomonas nitroreducens | |
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