Application | Comment | Organism |
---|---|---|
analysis | development of a coupled enzyme assays including 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide and phenazine methosulfate, resulting in the production of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide formazan which can be monitored at 570 nm | Streptomyces lividus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces fradiae |
expression in Escherichia coli | Streptomyces lividus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | Q53U15 | - |
- |
Streptomyces fradiae NCIMB 8233 | Q53U15 | - |
- |
Streptomyces lividus | Q2MF66 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
pH 7.5, temperature not specified in the publication | Streptomyces lividus |
6.4 | - |
pH 7.5, temperature not specified in the publication | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'''-N-acetyl-6'''-hydroxyneomycin C + O2 | about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C | Streptomyces fradiae | 2'''-N-acetyl-6'''-oxoneomycin C + H2O2 | - |
? | |
2'''-N-acetyl-6'''-hydroxyneomycin C + O2 | less than 10% of the rate with 6'''-deamino-6'''-hydroxyneomycin C | Streptomyces lividus | 2'''-N-acetyl-6'''-oxoneomycin C + H2O2 | - |
? | |
2'''-N-acetyl-6'''-hydroxyneomycin C + O2 | about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C | Streptomyces fradiae NCIMB 8233 | 2'''-N-acetyl-6'''-oxoneomycin C + H2O2 | - |
? | |
6'''-deamino-6'''-hydroxyneomycin C + O2 | - |
Streptomyces fradiae | 6'''-deamino-6'''-oxoneomycin C + H2O2 | - |
? | |
6'''-deamino-6'''-hydroxyneomycin C + O2 | - |
Streptomyces lividus | 6'''-deamino-6'''-oxoneomycin C + H2O2 | - |
? | |
6'''-deamino-6'''-hydroxyneomycin C + O2 | - |
Streptomyces fradiae NCIMB 8233 | 6'''-deamino-6'''-oxoneomycin C + H2O2 | - |
? | |
additional information | enzyme does not catalyze the reaction of EC 1.1.3.43, paromamine 6'-oxidase | Streptomyces lividus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LivQ | - |
Streptomyces lividus |
neoQ | - |
Streptomyces fradiae |