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Literature summary for 1.1.3.43 extracted from

  • Clausnitzer, D.; Piepersberg, W.; Wehmeier, U.F.
    The oxidoreductases LivQ and NeoQ are responsible for the different 6-modifications in the aminoglycosides lividomycin and neomycin (2011), J. Appl. Microbiol., 111, 642-651.
    View publication on PubMed

Application

Application Comment Organism
analysis development of a coupled enzyme assays including 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide and phenazine methosulfate, resulting in the production of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide formazan which can be monitored at 570 nm Streptomyces fradiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae Q53U15
-
-
Streptomyces fradiae NCIMB 8233 Q53U15
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
pH 7.5, temperature not specified in the publication Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paromamine + O2
-
Streptomyces fradiae 6'-dehydro-paromamine + H2O2
-
?
paromamine + O2
-
Streptomyces fradiae NCIMB 8233 6'-dehydro-paromamine + H2O2
-
?

Synonyms

Synonyms Comment Organism
neoQ
-
Streptomyces fradiae