BRENDA - Enzyme Database show
show all sequences of 1.1.3.42

Purification and N-terminal amino acid sequence of solanapyrone synthase, a natural Diels-Alderase from Alternaria solani

Katayama, K.; Kobayashi, T.; Chijimatsu, M.; Ichihara, A.; Oikawa, H.; Biosci. Biotechnol. Biochem. 72, 604-607 (2008)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, truncated solanapyrone synthase, SDS-PAGE
Alternaria solani
53000
-
gel filtration
Alternaria solani
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alternaria solani
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, preparative isoelectric focusing, and phenyl-Sepharose column chromatography
Alternaria solani
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00104
-
crude extract, pH 7.0, 30°C
Alternaria solani
1.69
-
after 1630 fold purification, pH 7.0, 30°C
Alternaria solani
Storage Stability
Storage Stability
Organism
-84°C, purified enzyme in 50 mM potassium phosphate buffer pH 7.0 in the presence of 30% (v/v) glycerol, 5 months, 14% loss of activity
Alternaria solani
Subunits
Subunits
Commentary
Organism
?
x * 41000, truncated solanapyrone synthase, SDS-PAGE
Alternaria solani
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
x * 41000, truncated solanapyrone synthase, SDS-PAGE
Alternaria solani
53000
-
gel filtration
Alternaria solani
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, preparative isoelectric focusing, and phenyl-Sepharose column chromatography
Alternaria solani
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00104
-
crude extract, pH 7.0, 30°C
Alternaria solani
1.69
-
after 1630 fold purification, pH 7.0, 30°C
Alternaria solani
Storage Stability (protein specific)
Storage Stability
Organism
-84°C, purified enzyme in 50 mM potassium phosphate buffer pH 7.0 in the presence of 30% (v/v) glycerol, 5 months, 14% loss of activity
Alternaria solani
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41000, truncated solanapyrone synthase, SDS-PAGE
Alternaria solani
Other publictions for EC 1.1.3.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741025
Kim
Functional analyses of the Die ...
Alternaria solani, Ascochyta rabiei
Mol. Plant Microbe Interact.
28
482-496
2015
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-
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2
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2
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2
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2
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2
-
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-
-
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-
714734
Kasahara
Solanapyrone synthase, a possi ...
Alternaria solani, Alternaria solani 548
ChemBioChem
11
1245-1252
2010
-
-
-
-
-
-
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6
-
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1
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1
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715308
Fujii
Functional analysis of fungal ...
Alternaria solani, Alternaria solani 548
J. Antibiot.
63
207-218
2010
-
-
1
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2
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5
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1
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4
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1
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1
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1
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1
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1
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4
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1
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1
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714520
Katayama
Purification and N-terminal am ...
Alternaria solani
Biosci. Biotechnol. Biochem.
72
604-607
2008
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-
-
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-
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-
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2
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2
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1
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2
1
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1
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2
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1
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2
1
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1
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714292
Katayama
Enzymatic activity and partial ...
Alternaria solani
Biochim. Biophys. Acta
1384
387-395
1998
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1
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1
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1
1
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2
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1
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2
2
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3
-
1
1
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1
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1
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1
-
1
1
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1
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2
2
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3
-
1
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