BRENDA - Enzyme Database show
show all sequences of 1.1.3.42

Enzymatic activity and partial purification of solanapyrone synthase: First enzyme catalyzing Diels-Alder reaction

Katayama, K.; Kobayashi, T.; Oikawa, H.; Honma, M.; Ichihara, A.; Biochim. Biophys. Acta 1384, 387-395 (1998)

Data extracted from this reference:

General Stability
General Stability
Organism
solanapyrone synthase activity is rather unstable in both crude and partially purified states. It loses 55% of its activity after 36 h at 4C but the addition of 30% glycerol markedly improves stability, the preparation retains 55% activity for 9 days at 4C. Other additives such as EDTA, thiol reagents and reducing agents are not effective
Alternaria solani
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
prosolanapyrone II
in 50 mM potassium phosphate buffer, pH 7.0, at 30C
Alternaria solani
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the activity of solanapyrone synthase is not affected by divalent cations
Alternaria solani
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
62000
native enzyme, gel filtration
Alternaria solani
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alternaria solani
-
-
-
Purification (Commentary)
Commentary
Organism
partial purification by ammonium sulfate precipitation and DEAE-Sepharose column chromatography
Alternaria solani
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.00084
-
crude extract, in 50 mM potassium phosphate buffer, pH 7.0, at 30C
Alternaria solani
0.0206
-
after 25fold purification, in 50 mM potassium phosphate buffer, pH 7.0, at 30C
Alternaria solani
Storage Stability
Storage Stability
Organism
4C, crude and partially purified states in the presence of 30% glycerol, 9 days, 45% loss of activity
Alternaria solani
4C, crude and partially purified states, 36 h, 55% loss of activity
Alternaria solani
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
in sodium acetate buffer
Alternaria solani
7
-
in potassium phosphate buffer
Alternaria solani
7.5
-
in Tris buffer
Alternaria solani
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3.7
-
irreversible inactivation is observed below pH 3.7
Alternaria solani
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the activity of solanapyrone synthase is not affected by NAD+, NADP+, FMN, and FAD+
Alternaria solani
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the activity of solanapyrone synthase is not affected by NAD+, NADP+, FMN, and FAD+
Alternaria solani
General Stability (protein specific)
General Stability
Organism
solanapyrone synthase activity is rather unstable in both crude and partially purified states. It loses 55% of its activity after 36 h at 4C but the addition of 30% glycerol markedly improves stability, the preparation retains 55% activity for 9 days at 4C. Other additives such as EDTA, thiol reagents and reducing agents are not effective
Alternaria solani
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
prosolanapyrone II
in 50 mM potassium phosphate buffer, pH 7.0, at 30C
Alternaria solani
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the activity of solanapyrone synthase is not affected by divalent cations
Alternaria solani
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
62000
native enzyme, gel filtration
Alternaria solani
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification by ammonium sulfate precipitation and DEAE-Sepharose column chromatography
Alternaria solani
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.00084
-
crude extract, in 50 mM potassium phosphate buffer, pH 7.0, at 30C
Alternaria solani
0.0206
-
after 25fold purification, in 50 mM potassium phosphate buffer, pH 7.0, at 30C
Alternaria solani
Storage Stability (protein specific)
Storage Stability
Organism
4C, crude and partially purified states in the presence of 30% glycerol, 9 days, 45% loss of activity
Alternaria solani
4C, crude and partially purified states, 36 h, 55% loss of activity
Alternaria solani
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
in sodium acetate buffer
Alternaria solani
7
-
in potassium phosphate buffer
Alternaria solani
7.5
-
in Tris buffer
Alternaria solani
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3.7
-
irreversible inactivation is observed below pH 3.7
Alternaria solani
Other publictions for EC 1.1.3.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741025
Kim
Functional analyses of the Die ...
Alternaria solani, Ascochyta rabiei
Mol. Plant Microbe Interact.
28
482-496
2015
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714734
Kasahara
Solanapyrone synthase, a possi ...
Alternaria solani, Alternaria solani 548
ChemBioChem
11
1245-1252
2010
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6
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715308
Fujii
Functional analysis of fungal ...
Alternaria solani, Alternaria solani 548
J. Antibiot.
63
207-218
2010
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5
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4
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1
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714520
Katayama
Purification and N-terminal am ...
Alternaria solani
Biosci. Biotechnol. Biochem.
72
604-607
2008
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2
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2
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1
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1
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714292
Katayama
Enzymatic activity and partial ...
Alternaria solani
Biochim. Biophys. Acta
1384
387-395
1998
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1
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3
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1
1
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1
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